发酵法制备萝卜硫素的工艺优化  

Optimization of Preparation of Sulforaphane by Fermentation

作　　者：阳晖[1] 卢凌霄 王晴 张川雨 曹沁倩 周先容[1] YANG Hui;LU Lingxiao;WANG Qing;ZHANG Chuanyu;CAO Qinqian;ZHOU Xianrong(School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100)

机构地区：[1]长江师范学院现代农业与生物工程学院,重庆408100

出　　处：《食品工业》2021年第12期218-223,共6页The Food Industry

基　　金：重庆市科委项目(cstc2019jcyjmsxmX0514);重庆市大学生创新创业训练项目(S202010647023);长江师范学院青年成才计划项目(2017QNRC13)资助。

摘　　要：以胭脂萝卜为原料,筛选合适的菌种发酵制备萝卜硫素。在单因素试验的基础上,以萝卜硫素的提取量为响应值,发酵温度、液料比、接种量以及pH为响应因子,对发酵工艺进行优化。结果表明:当发酵时间为96 h,发酵温度为31℃、液料比为3∶1(mL/g)、接种量为8.3%、pH为5.7时,选用黑曲霉发酵胭脂萝卜,萝卜硫素提取量最高,为0.5124±0.0065 mg/g,与模型预测值0.5131 mg/g接近。由此说明所建立的模型可以较好地预测各因素与萝卜硫素提取效果的关系,并能确定发酵法制备萝卜硫素的最佳发酵条件。Carmine radish was used as the raw material.And the suitable microbial strain was screened to produce sulforaphane.On the basis of single factor experiments,the fermentation process was optimized by response surface analysis with fermentation temperature,liquid-solid ratio,inoculum volume and pH as response factors and yield of sulforaphane as the response value.The results showed that best fermentation process was achieved under the conditions of microbial strain of Aspergillus niger:fermentation time of 96 h,fermentation temperature of 31℃,liquid-solid ratio of 3∶1(mL/g),inoculation amount of 8.3% and pH of 5.7.The highest yield of sulforaphane was 0.5124±0.0065 mg/g,which was basically consistent with the predicted value of model 0.5131 mg/g.This indicated that the model established not only could better predict the relationship between various factors and the yield of sulforaphane,but also could determine the best conditions of preparation of sulforaphane by fermentation.

关 键 词：胭脂萝卜 萝卜硫素 响应面 发酵 黑曲霉 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]