清香型大曲中产β-苯乙醇酵母的分离、鉴定及在白酒酿造中的应用  

Isolation and identification ofβ-phenylethanol-producing yeast from light-flavor Daqu and its application in Baijiu brewing

作　　者：张学林 季斌 许玲 张兴荣 贺连智 徐慧 李峰 黄艳红 ZHANG Xuelin;JI Bin;XU Ling;ZHANG Xingrong;HE Lianzhi;XU Hui;LI Feng;HUANG Yanhong(Shandong Food Ferment Industry Research and Design Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Shandong Bandaojing Co.,Ltd.,Zibo 256300,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省食品发酵工业研究设计院,山东济南250014 [2]山东扳倒井股份有限公司,山东淄博256300

出　　处：《中国酿造》2022年第1期123-127,共5页China Brewing

基　　金：2020科教产融合试点工程专项(2020KJC-ZD15)。

摘　　要：该研究采用传统培养分离法从清香型大曲中分离、筛选产β-苯乙醇酵母,通过形态观察及分子生物学技术对其进行菌种鉴定,并通过微量肉汤稀释法对其基因安全性进行评价,将其应用于白酒酿造。结果表明,分离、筛选到一株产β-苯乙醇的酵母菌株,编号为FJQC-XJ-4,经鉴定为库德里阿兹威(氏)毕赤酵母(Pichia kudriavzevii),该菌株具有较好的安全性。利用该菌株酿造白酒,使白酒中的β-苯乙醇(15.11 mg/L)、苯乙酸乙酯(5.35 mg/L)、乙酸异戊酯(23.76 mg/L)、乙酸乙酯(4.99 mg/L)和异丁醇含量(1.22 mg/L)分别提高12.83%、47.79%、3.66%、8.22%、29.06%,促进了白酒香气成分的生成;并使白酒粮香、窖香、糟香、酯香协调,口感醇甜、舒顺,改善了原酒的品质。Theβ-phenylethanol-producing yeast was isolated and screened from light-flavor Daqu by traditional culture and separation method,and identified by morphological observation and molecular biology techniques.The genetic safety of the screened strain was evaluated by microdilution broth method,and it was applied to Baijiu(Chinese liquor)brewing.The results showed that aβ-phenylethanol-producing yeast with good safety was isolated and screened,and number as FJQC-XJ-4,which was identified as Pichia kudriavzevii.The strain was used to brewing Baijiu,and the contents ofβ-phenylethanol(15.11 mg/L),ethyl phenylacetate(5.35 mg/L),isoamyl acetate(23.76 mg/L),ethyl acetate(4.99 mg/L)and isobutyl alcohol(1.22 mg/L)in the Baijiu increased by 12.83%,47.79%,3.66%,8.22%and 29.06%,respectively,which promoted the production of aroma components in Baijiu,and coordinated the aroma of grain,cellar,glutinous rice and ester of Baijiu.The taste of Baijiu was mellow,sweet and comfortable,which indicated that the quality of the original liquor was improved.

关 键 词：酵母 Β-苯乙醇 筛选 鉴定 应用 

分 类 号：TS264.2[轻工技术与工程—发酵工程]