信号分子AI-2对副溶血性弧菌四环素耐药基因接合转移的影响  

Effects of signaling molecule AI-2 on conjugation transfer of tetracycline resistance genes in Vibrio parahaemolyticus

作　　者：王婷 林才云[1,2] 姚琳 王联珠[2] 曲梦[2] 李风铃 谭志军[2,3] 江艳华[2] 王鹏 WANG Ting;LIN Cai-Yun;YAO Lin;WANG Lian-Zhu;QU Meng;LI Feng-Ling;TAN Zhi-Jun;JIANG Yan-Hua;WANG Peng(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Pilot National Laboratory for Marine Science and Technology,Qingdao 266033,China)

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003 [2]中国水产科学研究院黄海水产研究所农业农村部水产品质量安全检测与评价重点实验室,青岛266071 [3]青岛海洋科学与技术试点国家实验室,青岛266033

出　　处：《食品安全质量检测学报》2022年第2期505-510,共6页Journal of Food Safety and Quality

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022020004);国家自然科学基金项目(31601566);青岛市工程研究中心专项资金项目。

摘　　要：目的探讨信号分子AI-2对海产品中分离的副溶血性弧菌四环素耐药基因接合转移的影响作用。方法分别构建经AI-2处理和未经AI-2处理的副溶血性弧菌和大肠埃希氏菌的接合反应体系,通过菌落计数法测定不同浓度AI-2对副溶血性弧菌四环素耐药基因接合频率的影响,采用逆转录实时荧光定量聚合酶链式反应法测定不同浓度AI-2对接合转移调控相关基因转录水平的影响。结果副溶血性弧菌Vp2015094和Vp2016058的四环素耐药基因可以通过接合作用转移至大肠埃希氏菌K12中,15、30μmol/L浓度AI-2能显著提高Vp2015094的接合频率(P<0.05),6、15、30μmol/L浓度AI-2能极显著提高Vp2016058的接合频率(P<0.01),30μmol/L浓度AI-2能显著提高Vp2015094的接合转移调控相关基因tra F的转录水平以及Vp2016058的接合调控相关基因traF、trb B的转录水平(P<0.05)。结论AI-2能增强副溶血性弧菌四环素耐药基因的接合转移,表明AI-2能促进细菌耐药基因的扩散,为建立基于群体感应的细菌耐药性控制技术奠定基础。Objective To investigate the effects of signaling molecule AI-2 on conjugation transfer of tetracycline resistance genes in Vibrio parahaemolyticus strains from seafoods.Methods The conjugation reaction systems for Vibrio parahaemolyticus and Escherichia coli treated and untreated by AI-2 were constructed,the effects of AI-2 at different concentrations on conjugation frequency of tetracycline resistance genes in Vibrio parahaemolyticus were determined by colony counting method,the effects of AI-2 at different concentrations on the transcriptional levels of conjugation transfer related genes of Vibrio parahaemolyticus were assayed by reverse transcription and real-time quantitative polymerase chain reaction.Results The tetracycline resistance genes in Vibrio parahaemolyticus Vp2015094 and Vp2016058 could transfer into Escherichia coli K12,AI-2 at the concentrations of 15 and 30μmol/L could significantly increase the conjugation frequency of Vp2015094(P<0.05),AI-2 at concentrations of 6,15 and 30μmol/L could increase the conjugation frequency of Vp2016058 very significantly(P<0.01),30μmol/L AI-2 could significantly increase the transcriptional levels of conjugation transfer regulated genes traF in Vp2015094 and both traF and trbB in Vp2016058(P<0.05).Conclusion AI-2 can enhance the conjugation transfer of tetracycline resistance genes in Vibrio parahaemolyticus,which indicates that AI-2 can accelerate the dissemination of antibiotic resistance genes.These results provide a basis for developing control techniques of antibiotic resistance based on quorum sensing.

关 键 词：副溶血性弧菌 信号分子AI-2 四环素耐药基因 接合转移 

分 类 号：TS201.3[轻工技术与工程—食品科学]