姜黄素光动力技术对水产食品霍利斯格里蒙特菌和溶藻弧菌的灭活作用  

Inactivation of Curcumin Photodynamic Technology on Grimontia hollisae and Vibrio alginolyticus in Aquatic Food

作　　者：董冬丽 林少玲[2] 孙崇臻[1] 杜文琪 吴希阳[1] 唐书泽[1] Dong Dongli;Lin Shaoling;Sun Chongzhen;Du Wenqi;Wu Xiyang;Tang Shuze(Department of Food Science and Engineering,Jinan University,Guangzhou 510632;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]暨南大学食品科学与工程系,广州510632 [2]福建农林大学食品科学学院,福州350002

出　　处：《中国食品学报》2022年第2期40-48,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC1602504);闽对外合作项目(2020I0010);2018广东省重点领域研发计划项目(2019B020212004)。

摘　　要：目的:研究水溶性姜黄素介导的光动力技术对霍利斯格里蒙特菌和溶藻弧菌的灭活效果及其机理。方法:通过平板菌落计数法测定光动力技术对霍利斯格里蒙特菌和溶藻弧菌的灭活效果,采用试剂盒检测光动力作用后菌体内活性氧水平,利用十二烷基硫酸钠-聚丙稀酰胺凝胶电泳和琼脂糖凝胶电泳分析菌体蛋白降解和基因损伤程度。结果:水溶性姜黄素光动力对霍利斯格里蒙特菌和溶藻弧菌具有显著地灭活作用,其灭活效果随暗培养时间、姜黄素浓度和光照时间的增加而增强。光动力作用后,霍利斯格里蒙特菌和溶藻弧菌分别降低7.98 lg(CFU/mL)和7.80 lg(CFU/mL),灭活率高达99.99%以上。姜黄素光动力作用诱导霍利斯格里蒙特菌和溶藻弧菌体内产生高水平的活性氧,破坏菌体蛋白和DNA,而对霍利斯格里蒙特菌和溶藻弧菌的毒力基因损伤作用不明显。结论:姜黄素光动力技术通过激发活性氧有效灭活霍利斯格里蒙特菌和溶藻弧菌,在水产食品安全防控领域具有良好的应用前景。Objectives:To investigate photodynamic technology(PDT)inactivation of Grimontia hollisae(Gh)and Vibrio alginolyticus(Va)mediated by water soluble curcumin and its mechanism.Methods:The inactivation effect of Gh and Va by PDT was determined by colony counting method,reactive oxygen species(ROS)production in bacteria was tested by ROS assay kit,total protein degradation was analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and virulence gene damage was examined by agarose gel electrophoresis.Results:Water soluble curcumin PDT significantly inactivated Gh and Va.The inactivation effects increased with dark incubation time,illumination time and curcumin concentration.After PDT treatment,Gh and Va decreased by 7.98 lg(CFU/mL)and 7.80 lg(CFU/mL),and the inactivation rate was over 99.99%.PDT promoted the ROS level in bacteria to degrade bacteria protein and DNA,but no significant damage to virulence genes of Gh and Va was observed.Conclusion:Curcumin PDT can effectively inactivate Gh and Va via ROS and shows potential application in aquatic food safety.

关 键 词：光动力技术 水溶性姜黄素 水产食品 霍利斯格里蒙特菌 溶藻弧菌 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]