MicroRNA-382 inhibits the proliferation of mouse spermatogoniaby targeting Kmt5a  

作　　者：YI ZHENG PENGFEI ZHANG JINSHENG ZHU LINGKAI ZHANG WENXIAN ZENG 

机构地区：[1]Key Laboratory for Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling,712100,China

出　　处：《BIOCELL》2020年第2期201-207,共7页生物细胞（英文）

基　　金：the National Natural Science Foundation of China(http://www.nsfc.gov.cn/,Grant No.31572401)to W.Z.,the Natural Science Foundation of Shaanxi Province;China(http://kjt.shaanxi.gov.cn/,Grant No.2019JQ-430);Young Talent fund of University Association for Science and Technology in Shaanxi,China(http://www.snast.org.cn/,Grant No.20180204);China Postdoctoral Science Foundation(http://jj.chinapostdoctor.org.cn/website/index.html,Grant No.2018M641032);a startup fund from Northwest A&F University(https://www.nwafu.edu.cn/,Grant No.2452018037)to Y.Z.Conflicts of Interest:The authors declare that they have no conflict of interest.

摘　　要：Spermatogenesis is a highly efficient and intricate process in the testis by which mature spermatozoa are produced daily to maintain lifelong male fertility.Essential to this process are spermatogonia capable of both proliferation and differentiation.Nevertheless,the underlying mechanisms for spermatogonial proliferation and differentiation remain poorly understood.MicroRNAs(miRNAs)are a category of non-coding small RNAs with regulatory functions by binding to the 3’untranslated region(UTR)of the target mRNA.Previous studies have demonstrated that miRNAs are capable of modulating cell proliferation,differentiation and apoptosis,but the roles of individual miRNAs in spermatogonial fate determination remain largely elusive.Here,by using a mouse spermatogonial cell line(GC-1),we investigated the role for miRNA-382 in spermatogonial proliferation.We found that pre-miRNA-382 was expressed in spermatogonia.The luciferase reporter assay demonstrated Kmt5a but not Top1 as a target gene of miRNA-382.Overexpression of miRNA-382 by transfecting a miRNA mimic downregulated Kmt5a at both RNA and protein levels,and further reduced the proliferation and viability of spermatogonia.Knockdown of Kmt5a by RNA interference(RNAi)resulted in a uniform phenotype in spermatogonia.We therefore conclude that miRNA-382 inhibits the proliferation of mouse spermatogonia by targeting Kmt5a.Our finding extends the knowledge about the regulatory roles of miRNAs in spermatogonia and lays the groundwork for diagnosis and treatment of male infertility.

关 键 词：SPERMATOGENESIS SELF-RENEWAL TESTIS 

分 类 号：R73[医药卫生—肿瘤]