碎米荠硒肽的制备及其体外抗氧化活性分析  

Preparation and Antioxidant Activity of Se-containing Peptides from Cardamine enshiensis in Vitro

作　　者：贾蕾 何慧[1] 向极钎 赵小红 侯焘[1] JIA Lei;HE Hui;XIANG Jiqian;ZHAO Xiaohong;HOU Tao(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Enshi Tujia&Miao Autonomous Prefecture Academy of Agricultural Sciences,Enshi 445002,China)

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]恩施土家族苗族自治州农业科学院,湖北恩施445002

出　　处：《食品工业科技》2022年第6期205-212,共8页Science and Technology of Food Industry

基　　金：中国博士后科学基金第67批面上资助(2020M672313)。

摘　　要：本文以恩施碎米荠为原料,采用酶法制备具有抗氧化活性的碎米荠硒肽。以蛋白提取率为指标,通过单因素实验和正交试验优化了碎米荠蛋白的提取条件;以DPPH·、ABTS^(+)·清除率为考察指标,优化了碎米荠蛋白的酶解参数,利用超滤设备对碎米荠蛋白酶解液进行分级分离,确定了碎米荠硒肽制备的最佳方案;考察了碎米荠硒肽的还原力、Fe^(2+)螯合能力。结果表明,碎米荠蛋白提取的最优条件为:提取溶液0.1 mol/L NaOH、液料比45:1 mL/g、提取温度60℃、提取时间3 h、提取2次,此时碎米荠蛋白的提取率为72.91%。碎米荠硒肽制备的最佳条件为:碱性蛋白酶、底物浓度4%、酶底比1.0%、酶解时间1 h,超滤膜截留分子量为1 kDa,所得碎米荠硒肽的含硒量为362.378 mg/kg,其DPPH·、ABTS^(+)·清除率的EC50值分别为0.435和0.399 mg/mL,并表现出良好的还原力和Fe^(2+)螯合能力。In this study,the enzymatic method was used to prepare Se-containing peptides(SeCPPs)with antioxidant activity from Cardamine enshiensis.The extraction conditions of protein of C.enshiensis(SeCP)were optimized by single-factor experiments and orthogonal test with protein extraction rate as the index.The enzymatic parameters of SeCP were optimized with DPPH· and ABTS^(+)· scavenging rate as the investigated index,and ultrafiltration equipment was used to determine the best protocol for the SeCPPs.The reducing power and Fe^(2+)chelating ability of Se-containing peptides were investigated.The results showed that the optimal conditions for the extraction of SeCP were:0.1 mol/L NaOH solution,liquid to material ratio 45:1 mL/g,extraction temperature 60°C,extraction time 3 h,twice.Under this condition,the extraction rate of SeCP was 72.91%.The optimum conditions for the enzymatic preparation of antioxidant SeCPPs were:4%substrate concentration,1.0%enzyme substrate ratio,1 h digestion time,1 kDa molecular weight retained by ultrafiltration membrane.The Se content in SeCPP was 362.378 mg/kg,and the EC50 values of DPPH· and ABTS^(+)· scavenging rate were 0.435 and 0.399 mg/mL,respectively.Besides,SeCPPs showed better reducing power and Fe^(2+)chelating ability.

关 键 词：恩施碎米荠 抗氧化肽 酶解 超滤 

分 类 号：TS201.2[轻工技术与工程—食品科学]