异硫氰酸苄酯对于黄曲霉生长速率和产毒情况的影响  

Effects of Benzyl Isothiocyanate on Growth and Toxin Production of Aspergillus flavus

作　　者：程月 朱姗姗 刘静[2] 张世凯 翟晓松 刘阳[1] 赵月菊[1] CHENG Yue;ZHU Shanshan;LIU Jing;ZHANG Shikai;ZHAI Xiaosong;LIU Yang;ZHAO Yueju(Institute of Agricultural Products Processing,Chinese Academy of Agricultural Sciences,Beijing 100193;College of Food and Science Engineering,Shandong Agricultural University,Tai'an,Shandong 271018)

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]山东农业大学食品与科学工程学院,山东泰安271018

出　　处：《核农学报》2022年第1期114-120,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFC1600905)。

摘　　要：为明确异硫氰酸苄酯(BITC)在不同条件下对黄曲霉的抑制效果,本研究以熏蒸法,在28℃培养条件下,分别以花生和玉米为培养基质,研究不同浓度(0、5、10、15、20 mg·L^(-1))异硫氰酸苄酯在不同水分活度(a_(w))(0.930、0.960、0.980、0.995)下对黄曲霉(Aspergillus flavus)生长和产毒情况的影响。结果表明,BITC能显著影响黄曲霉的生长和产毒,且影响程度与BITC浓度、培养基质和a_(w)密切相关。BITC浓度增加和a_(w)降低均能显著增强BITC的抑菌活性。除在a_(w)为0.930的花生培养基质外,BITC均可显著抑制黄曲霉的生长和产毒且抑制效果与其浓度成正比。相比于高水分活度,BITC在低水分活度(a_(w)=0.930)条件下,对黄曲霉抑制效果更强。本研究为进一步开发基于BITC的抑菌产品提供了理论支撑。To study the inhibition effect of benzyl isothiocyanate(BITC)on Aspergillus flavus(A.flavus)under different conditions,the effect of BITC(0,5,10,15 and 20 mg·L^(-1))on growth rate and aflatoxin B1(AFB1)production of A.flavus under different water activity(aw)(0.995,0.980,0.960 and 0.930)at 28℃based on peanuts and corn media,respectively.The presence of BITC affected growth and AFB1 production of A.flavus,and this effect depended on the dose,matrix and a_(w).Increased concentration of BITC and decreased a_(w)significantly enhanced the inhibitory activity of BITC.BITC significantly inhibited the growth and AFB1 production of A.flavus,and the inhibition effect was proportional to its dose,except for peanut media with a_(w)of 0.995.When a_(w)was 0.930,the minimum inhibitory concentrations of BITC for the peanut and corn media were the lowest.This study provides theoretical support for further development of antifungal products based on BITC.

关 键 词：异硫氰酸苄酯 黄曲霉 AFB 水分活度 培养基 

分 类 号：TS201.6[轻工技术与工程—食品科学]