响应面法优化菌体蛋白水解工艺制备有机氮源的研究  

Optimization of microbial proteolysis process to produce organic nitrogen by response surface methodology

作　　者：孙科[1] 王萌 冯希勇 SUN Ke;WANG Meng;FENG Xiyong(Department of Medicine and Food,Xuzhou Engineering Vocational and Technical College,Xuzhou 221006)

机构地区：[1]徐州生物工程职业技术学院,药品食品学院,徐州221006

出　　处：《中国食品添加剂》2022年第3期83-91,共9页China Food Additives

基　　金：徐州市科技计划项目(编号:KC20051);江苏高校“青蓝工程”资助基金项目(编号:JS1111)。

摘　　要：利用联合法对柠檬酸发酵废菌体进行细胞破壁处理,通过单因素试验和响应面试验方法对菌体蛋白水解条件进行优化,最终得到菌体蛋白水解最优工艺条件组合为:p H3.0、反应温度为56℃、反应时间为15.5h、酸性蛋白酶添加量为2.5g·100g^(-1),在此最优水解条件下,水解产物中总氮达到0.82g·100g^(-1)、氨基酸态氮达到0.46g·100g^(-1)。以菌体蛋白水解产物为有机氮源进行坚强芽孢杆菌Ba-2培养,结果显示菌体蛋白水解产物和酵母浸提物效果相当,为发酵废菌体的工业化利用开辟了新的路径。Cell walls of citric acid fermentation residues were broken down and proteolysis conditions were optimized through single factor and response surface method.The optimal of cell proteolysis conditions were:p H value 3.0,reaction temperature 56℃,reaction time 15.5h,and the amount of acid protease 2.5g·100g^(-1).Under the optimal hydrolysis conditions,the total nitrogen in hydrolyzate reached 0.82g·100g^(-1),and the amino acid nitrogen reached0.46g·100g^(-1).The microbial protein hydrolysate was used as the organic nitrogen source for cultivating of Bacillus firmus Ba-2.The results showed that the microbial protein hydrolysate and yeast extract had the same effect,which provides a new path for the industrial utilization of fermentation waste.

关 键 词：发酵废菌体 菌体蛋白 水解 有机氮源 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS201.3[轻工技术与工程—食品科学与工程]