雪菊精油对希氏肠球菌产酪胺及相关基因表达的影响  

Effect of Coreopsis tinctoria Essential Oil on Tyramine Production and Related Gene Expression in Enterococcus hirae

作　　者：李亚倬 黄亚丽 于红红 王静云[1] 卢士玲[1] LI Yazhuo;HUANG Yali;YU Honghong;WANG Jingyun;LU Shiling(School of Food Science and Technology,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学食品学院,新疆石河子832000

出　　处：《食品科学》2022年第6期104-111,共8页Food Science

基　　金：国家自然科学基金地区科学基金项目(32060547)。

摘　　要：探讨不同质量浓度雪菊精油对希氏肠球菌(Enterococcus hirae)N47产酪胺的影响机制。利用反转录实时定量聚合酶链式反应分析E.hirae在雪菊精油作用下酪氨酸脱羧途径相关基因的表达情况;利用高效液相色谱法检测不同质量浓度雪菊精油对E.hirae产酪胺的影响。并将E.hirae接入到含不同质量浓度雪菊精油的熏马肠中发酵,评估香肠pH值、菌落总数和酪胺积累量。结果表明:在E.hirae纯培养体系和熏马肠体系中,雪菊精油通过抑制微生物的生长和酪氨酸脱羧途径中tyr DC、tyr P基因的表达,降低酪胺的积累量(P<0.05)。当雪菊精油添加量为1/2最小抑菌浓度(minimal inhibitory concentration,MIC)和MIC时,熏马肠中酪胺的含量分别为78.52 mg/kg和45.83 mg/kg,较对照组分别减少了64.72%和79.41%。The aim of this study was to investigate the effect of different concentrations of Coreopsis tinctoria essential oil on tyramine production by Enterococcus hirae N47.Reverse transcription quantitative real-time polymerase chain reaction(real-time PCR)was used to analyze the expression of the genes involved in the tyrosine decarboxylase(TDC)pathway in E.hirae treated with C.tinctoria essential oil.High performance liquid chromatography(HPLC)was used to detect tyramine.The changes in pH,total viable count and tyramine accumulation in smoked horse sausage with different concentrations of C.tinctoria essential oil inoculated with E.hirae were evaluated during the 28-day fermentation period.The results showed that for both the pure culture of E.hirae and smoked horse sausage,C.tinctoria essential oil significantly reduced tyramine accumulation by inhibiting the growth of microorganisms and the expression of the tyr DC and tyr P genes in the tyrosine decarboxylation pathway(P<0.05).Tyramine contents in smoked horse sausage with C.tinctoria essential oil at the minimal inhibitory concentration(MIC)and 1/2 MIC were 78.52 and 45.83 mg/kg,respectively,which were 64.72%and 79.41%lower than that in the control group(not added with C.tinctoria essential oil,inoculated with E.hirae),respectively.

关 键 词：雪菊精油 酪胺 希氏肠球菌 酪氨酸脱羧途径 基因表达 

分 类 号：TS207.7[轻工技术与工程—食品科学]