特香型白酒大曲中一株耐热产淀粉酶地衣芽孢杆菌的分离和鉴定  

Isolation and Identification of a Heat-Resistant Amylase-Producing Bacillus licheniformis Strain from Texiang Daqu

作　　者：游勇 吴晓玉 廖家富[2] 吴生文 廖昶[3] 王飞[1,2] 胡余粮 林钰宽[1,2] 孙辉凡 丁忠涛 YOU Yong;WU Xiaoyu;LIAO Jiafu;WU Shengwen;LIAO Chang;WANG Fei;HU Yuliang;LIN Yukuan;SUN Huifan;DING Zhongtao(Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,Nanchang,Jiangxi 330045;College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang,Jiangxi 330045;Sitir Liquor Co.Ltd.,Zhangshu,Jiangxi 331200,China)

机构地区：[1]江西省农业微生物资源开发与利用工程实验室,江西南昌330045 [2]江西农业大学生物科学与工程学院,江西南昌330045 [3]四特酒有限责任公司,江西樟树331200

出　　处：《酿酒科技》2022年第3期17-24,共8页Liquor-Making Science & Technology

基　　金：江西省自然科学基金项目(20192BAB214006);江西省教育厅科学技术研究项目(GJJ170311);企业合作项目;国家级大学生创新训练计划项目(202110410035)。

摘　　要：耐高温的细菌是中高温大曲的重要优势菌,为了鉴定特香型白酒大曲的功能细菌,从特香型大曲火圈层中分离筛选到1株具有水解淀粉活性的耐高温细菌菌株ZRF-1。本试验结合扫描电镜鉴定菌株形态、生理生化特性鉴定和gyrB基因序列同源性分析鉴定该菌株为地衣芽孢杆菌。利用该菌株模拟大曲制作工艺固态发酵产生吡嗪类风味化合物四甲基吡嗪、2,3,5-三甲基吡嗪和2,5-二甲基吡嗪,以及大量的乙偶姻。ZRF-1菌株在产酶培养基中摇瓶发酵的淀粉酶活力可达53.42 U/mL。初步确定地衣芽孢杆菌ZRF-1菌株为特香型大曲的功能菌株,可以丰富白酒酿造的菌种资源库。Heat-resistant bacteria are important dominant bacteria in medium and high temperature Daqu.In order to identify the func-tional bacteria of Texiang Daqu,a heat-resistant strain ZRF-1 with starch hydrolysis activity was isolated from the fire circle layer of Texiang Daqu.By using scanning electron microscopy,physiological and biochemical experiments,and gyrB gene sequence homolo-gy analysis,the strain was identified as Bacillus licheniformis.The strain was used in the simulated solid-state fermentation process of Daqu,and pyrazine flavor compounds,including tetramethylpyrazine,2,3,5-trimethylpyrazine and 2,5-dimethylpyrazine,and large amounts of acetoin were produced.The amylase activity of strain ZRF-1 was 53.42 U/mL in enzyme-producing medium by shake flask culture.It is preliminarily determined that the Bacillus licheniformis strain ZRF-1 is a functional strain of Texiang Daqu,which expands the strain resource repository of Baijiu production.

关 键 词：特香型大曲 地衣芽孢杆菌 淀粉酶活力 吡嗪类风味化合物 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]