方竹笋膳食纤维作为益生元对乳酸菌生长的影响  

Effect of bamboo shoot(Chimonobambusa quadrangularis)dietary fiber as prebiotics on the growth of lactic acid bacteria

作　　者：赵泓舟 杜木英[1] ZALAN Zsolt HEGYI Ferenc 吴良如 张同芳 郑炯[1] ZHAO Hongzhou;DU Muying;ZALAN Zsolt;HEGYI Ferenc;WU Liangru;ZHANG Tongfang;ZHENG Jiong(College of Food Science,Southwest University,Chongqing 400715,China;Food Science and Technology Institute,Hungarian University of Agriculture and Life Sciences,Budapest 1011-1239,Hungarian;China National Bamboo Research Center,Hangzhou 310012,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]匈牙利农业与生命科学大学,食品科学与技术研究所,布达佩斯1011-1239 [3]国家林业和草原局竹子研究开发中心,浙江杭州310012

出　　处：《食品与发酵工业》2022年第6期174-180,共7页Food and Fermentation Industries

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0392);重庆市林业局科技兴林项目(2021-8);贵州省特色林业产业研发项目(2020-28);中央高校基本科研业务费重点项目(XDJK2020B045)。

摘　　要：为了探究竹笋膳食纤维(bamboo shoot dietary fiber,BSDF)作为益生元对乳酸菌生长的影响,以常见益生元菊粉为对照,检测了BSDF在模拟胃肠液中的抗消化性,以及对乳酸菌L1(Lactobacillus fermentum)、L2(Lactobacillus plantaum)生长过程中pH值、总酸含量和生长曲线的影响。结果表明,BSDF中总膳食纤维、不溶性膳食纤维、可溶性膳食纤维含量分别为64.90%、58.12%、6.78%,相对含量较高的单糖有葡萄糖(39.72%)、阿拉伯糖(12.77%)、木糖(12.69%)。BSDF在胃肠液中具有一定的抗消化性,模拟消化6 h后,BSDF在人工胃液(pH=3.5)和人工肠液(pH=7)的水解率分别为15.54%、31.08%,均低于菊粉对照组。对于乳酸菌L1,随着BSDF添加量的增加,培养基中的菌群浓度不断增加,BSDF相比于菊粉更有利于乳酸菌L1生长。对于乳酸菌L2,随着BSDF添加量的增加,总酸含量逐渐增大,乳酸菌的益生效应也随之增强。该研究可为BSDF作为益生元的开发利用提供理论参考。To explore the effect of bamboo shoot(Chimonobambusa quadrangularis)dietary fiber(BSDF)as prebiotics on the growth of lactic acid bacteria,the anti-digestibility of BSDF in simulated gastric and intestinal fluid,and the impact of BSDF on pH value,total acid content and growth curve of Lactobacillus fermentum(L1)and Lactobacillus plantaum(L2)during growth were investigated with inulin as control.The results showed that the total dietary fiber,insoluble dietary fiber,and soluble dietary fiber content in BSDF was 63.90%,58.12%,and 6.78%,respectively,and the monosaccharides with relatively higher content were glucose(39.72%),arabinose(12.77%),and xylose(12.69%).BSDF had anti-digestibility in gastric and enteric fluid,and the hydrolysis rate of BSDF after simulated digestion in gastric fluid(pH=3.5)and enteric fluid(pH=7)for 6 h was 15.54%and 31.08%,respectively,lower than that of inulin.For L1,the concentration of bacteria in the medium increased with the increase of BSDF addition.BSDF was more beneficial to L1 growth than inulin.For L2,with the increase of BSDF,the total acid content increased,and the probiotic effect of lactic acid bacteria was also enhanced.This study can provide theoretical reference for BSDF as a prebiotic on lactic acid bacteria.

关 键 词：竹笋膳食纤维 乳酸菌 抗消化实验 益生效应 生长曲线 

分 类 号：TS201.3[轻工技术与工程—食品科学]