库尔勒香梨果实发育过程中酚类物质及抗氧化活性研究  

Phenolic compounds and antioxidant activity of Kuerlexiangli pear fruit during the growth period

作　　者：仵菲 蒲云峰[2] 雷晓钰 何志浩 沈立荣[3] 白红进[1] WU Fei;PU Yunfeng;LEI Xiaoyu;HE Zhihao;SHEN Lirong;BAI Hongjin(College of Life Sciences,Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Xin-jiang Production&Construction Corps,Alar 843300,Xinjiang,China;College of Food Science and Engineering,Tarim University,Alar 843300,Xinjiang,China;School of Biosystems Engineering&Food Science,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]塔里木大学生命科学学院·新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [2]塔里木大学食品科学与工程学院,新疆阿拉尔843300 [3]浙江大学生物系统工程与食品学院,杭州310058

出　　处：《果树学报》2022年第4期574-583,共10页Journal of Fruit Science

基　　金：兵团重大科技项目(2017DB006);兵团南疆重点产业支撑计划项目(2017DB006-4)。

摘　　要：【目的】探讨库尔勒香梨发育过程中不同果实部位的酚类物质含量及抗氧化活性的动态变化趋势。【方法】对库尔勒香梨果实生长发育过程中不同组织部位的总酚、总黄酮、酚类物质含量及其对ABTS+自由基清除能力、DPPH自由基清除能力和铁还原能力(FRAP)进行测定,并分析酚类物质与抗氧化能力之间的相关性。【结果】在库尔勒香梨果实生长发育过程中果皮、果肉和心外果肉中总黄酮、总酚含量、DPPH自由基清除能力、FRAP和ABTS+自由基清除能力均呈现先上升后下降的趋势;在果皮中熊果苷含量最高,在果肉和心外果肉中只检测出熊果苷、绿原酸、儿茶素、表儿茶素、异鼠李糖-3-O-芸香糖苷和异鼠李素-3-O-葡萄糖苷;在香梨果心中总黄酮、总酚含量和FRAP呈现逐渐下降的趋势,DPPH自由基清除能力、ABTS+自由基清除能力呈现先上升后下降的趋势,且绿原酸含量最高;库尔勒香梨果实中酚类物质的抗氧化活性极高,其中与FRAP呈显著正相关(p<0.01)。【结论】库尔勒香梨果实含有丰富的酚类物质,随着果实的发育呈现逐渐降低的趋势。果皮和果心具有更高含量的酚类物质和更强的抗氧化能力。【Objective】To investigate the dynamic changes of phenolic content and antioxidant activity of different parts of the fruits of Kuerlexiangli pear during the development.【Methods】In this paper,the content of total phenols, total flavonoids, phenolic substances and their ability to scavenge ABTS+free radicals, DPPH free radical scavenging ability and iron reducing ability(FRAP) were measured in different tissue parts during the growth and development of the Kuerlexiangli pear fruits, and the correlations between phenolic substances and antioxidant capacity were analyzed.【Results】The total flavonoids, total phenols, DPPH radical scavenging capacity, FRAP iron-reducing capacity and ABTS+radical scavenging capacity in the peel, flesh and extra-kernel flesh of the Kuerlexiangli pear showed an increasing and then decreasing trend during the growth and development of the fruits. The arbutin, chlorogenic acid, catechin, epicatechin, isorhamnose-3-O-rutinoside and isorhamnose-3-O-glucoside were detected in the flesh and extra-kernel flesh, the content of the arbutin was highest in the peel. The total flavonoids, total phenols and FRAP iron reduction capacity showed a decreasing trend in the heart of the Kuerlexiangli pear, while DPPH radical scavenging capacity and ABTS+radical scavenging capacity showed an increasing and then decreasing trend, and the content of chlorogenic acid was highest. The antioxidant activity of phenolic substances in the fruits of the Kuerlexiangli pear was extremely high,and it was significantly positively correlated with FRAP iron reduction capacity(p < 0.01).【Conclusion】The fruits of the Kuerlexiangli pear were rich in phenolic substances, which showed a gradual decrease with the development of the fruits. The peel and kernel had a higher content of phenolic substances and a stronger antioxidant capacity.

关 键 词：库尔勒香梨 总黄酮 总酚 酚类物质 抗氧化活性 

分 类 号：S661.2[农业科学—果树学]