2种蛋白肽对电离辐射损伤小鼠的保护作用  

Radioprotective Effect of Two Kinds of Protein Peptides in Rayirradiated Mice

在线阅读下载全文

作  者:刘爱青 王海燕 王应强[3] 闫征 李志国 许智敏 LIU Aiqing;WANG Haiyan;WANG Yingqiang;YAN Zheng;LI Zhiguo;XU Zhimin(Beijing Semnl Biotechnology Co.,Ltd.,Beijing 100062,China;Anhui Semnl Biotechnology Co.,Ltd.,Huaibei 235000,China;College of Agriculture and Forestry,Longdong University,Qingyang 745000,China)

机构地区:[1]北京盛美诺生物技术有限公司,北京100062 [2]安徽盛美诺生物技术有限公司,安徽淮北235000 [3]陇东学院,农林科技学院,甘肃庆阳745000

出  处:《食品工业科技》2022年第9期359-364,共6页Science and Technology of Food Industry

摘  要:目的:探究众多生物活性肽中口感愉悦的菠萝蜜蛋白肽和胶原蛋白肽对60钴γ射线辐射损伤小鼠的辅助保护作用。方法:采用无特定病原体(SPF级)雌性BALB/c小鼠288只,随机分为四批,每批6组,每组12只。每组设计空白对照组、模型对照组、菠萝蜜蛋白肽低、高剂量组(0.20、0.80 g/kg BW)以及胶原蛋白肽低、高剂量组(0.53、1.6 g/kg BW)。每日一次经口给予小鼠受试品26 d后,除空白对照组,其它各组接受;钴γ射线全身辐射,辐射后继续灌胃干预。分别检测外周血白细胞数(照射剂量3.5 Gy,照射前、照射后第3 d、第14 d)、骨髓细胞DNA含量和血清8羟基脱氧鸟苷的含量(照射剂量3.5 Gy,照射后第3 d)、血清溶血素含量(照射剂量1 Gy,照射后第7 d),血中超氧化物歧化酶(SOD)活性(照射剂量7 Gy,照射后第7 d)。结果:与模型对照组比较,照射后第3 d、菠萝蜜蛋白肽高剂量组、胶原蛋白肽低、高剂量组外周血白细胞数明显升高(P<0.05),照射后第14 d,菠萝蜜蛋白肽高剂量组、胶原蛋白肽高剂量组外周血白细胞数显著升高(P<0.05);照射后第3 d,菠萝蜜蛋白肽高剂量组能显著升高小鼠骨髓细胞DNA含量(P<0.05),菠萝蜜蛋白肽高剂量组和胶原蛋白肽高剂量组能显著降低辐照后小鼠血清中8羟基脱氧鸟苷的含量(P<0.01);照射后第7 d菠萝蜜蛋白肽高剂量组能显著升高小鼠血清溶血素的含量(P<0.05)。结论:菠萝蜜蛋白肽对电离辐射损伤有辅助保护作用,胶原蛋白肽对电离辐射损伤的小鼠外周血白细胞数具有一定的改善作用,且能降低辐射后小鼠血清中8羟基脱氧鸟苷的含量。Objective:To investigate the radioprotective effect of jackfruit protein peptide and collagen peptide with pleasant mouthfeel in many bioactive peptides in;Co γ ray-irradiated mice.Methods:Two hundred and eighty eight specific pathogen free(SPF) female BALB/c mice were randomly divided into 4 batches with 6 groups and 12 rats in each group.Each batch divided into blank control group,model control group,2 jackfruit protein peptide intervention groups(0.20,0.80 g/kg BW) and 2 collagen peptide intervention groups(0.53,1.6 g/kg BW).After the 26 days of the intragastric administration,the mice except the blank control group received;Co γ-ray whole body irradiation,and intragastric intervention continued after irradiation.The number of peripheral blood white blood cells(radiation dose 3.5 Gy,3 and 14 days after irradiation),bone marrow DNA content and the content of 8-OhdG in serum(radiation dose 3.5 Gy,3 days after radiation),serum hemolysin(radiation dose 1 Gy,7 days after radiation) and superoxide dismutase(SOD) activity in blood(radiation dose 7 Gy,7 days after radiation) were measured,respectively.Results:Compared with the model control group,the number of peripheral blood white blood cells in high dose of jackfruit peptide,and low and high dose of collagen peptide were significantly increased on 3rd day after radiation(P<0.05).The number of peripheral blood white blood cells in high dose of jackfruit peptide,and high dose of collagen peptide were significantly increased on 14th day after radiation(P<0.05).Bone marrow DNA content in high dose of jackfruit peptide were significantly increased on 3rd day after radiation(P<0.05).The high-dose group of jackapple peptide and the high-dose group of collagen peptide could significantly reduce the 8-hydroxydeoxyguanosine content in mouse serum after irradiation(P<0.01).Serum hemolysin were significantly increased on 7th day after radiation(P<0.05).Conclusion:Jackfruit peptide had protective effects against ionizing radiation exposure.Collagen peptides improved the numbe

关 键 词:菠萝蜜蛋白肽 胶原蛋白肽 小鼠 辐射危害 保护 

分 类 号:TS202.1[轻工技术与工程—食品科学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象