酶辅助三相分离法同时提取亚麻籽油、亚麻籽蛋白和亚麻籽胶工艺优化  

Optimization of simultaneous extraction of oil, protein and gum from flaxseed by enzyme-assisted three phase partitioning

作　　者：姚思含 廖敏和 康佳欣 魏子凯 刘宁[1,2,3] 任皓威 YAO Sihan;LIAO Minhe;KANG Jiaxin;WEI Zikai;LIU Ning;REN Haowei(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Key Laboratory of Dairy Science of Ministry of Education,Northeast Agricultural University,Harbin 150030,China;Harbin Tengning Technology Co.,Ltd.,Harbin 150010,China)

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]东北农业大学教育部乳品重点实验室,哈尔滨150030 [3]哈尔滨腾凝科技有限公司,哈尔滨150010

出　　处：《中国油脂》2022年第3期11-17,共7页China Oils and Fats

摘　　要：采用酶辅助三相分离法(EATPP)同时提取亚麻籽中的油脂、蛋白质和胶,以亚麻籽油提取率为指标,通过单因素实验和响应面实验优化EATPP工艺条件。结果表明,最优EATPP工艺条件为酶解时间2 h,酶添加量433 U/g,叔丁醇用量3.9 mL/g,硫酸铵用量2.1 g/g,三相提取温度45℃,三相提取时间4 h。在最优工艺条件下,3个品种亚麻籽的亚麻籽油提取率为84.47%~89.03%,亚麻籽蛋白提取率为50.58%~55.69%,亚麻籽胶提取率为31.62%~35.61%。采用EATPP不仅能够同时分离亚麻籽中脂肪、蛋白质和胶,而且能够降低成本和提高亚麻籽的利用率。Enzyme-assisted three phase partitioning(EATPP) was used to simultaneously extract oil, protein and gum from flaxseed, and the extraction rate of flaxseed oil was used as an indicator to optimize the process conditions of EATPP by single factor experiment and response surface methodology. The results showed that the optimal EATPP process conditions were obtained as follows: enzymatic hydrolysis time 2 h, enzyme dosage 433 U/g, tert-butanol dosage 3.9 mL/g, ammonium sulfate dosage 2.1 g/g, three phase extraction temperature 45℃, three phase extraction time 4 h. Under the optimal process conditions, the extraction rate of the flaxseed oil from three varieties reached 84.47%-89.03%, the extraction rate of flaxseed protein reached 50.58%-55.69%, and the extraction rate of flaxseed gum reached 31.62%-35.61%. Using EATPP can not only simultaneously extract oil, protein and gum from flaxseed, but also can reduce cost and improve the utilization rate of flaxseed.

关 键 词：亚麻籽油 亚麻籽蛋白 亚麻籽胶 三相分离法 水酶法 同时提取 

分 类 号：TS224[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ644[轻工技术与工程—食品科学与工程]