Synergy effects of Asperosaponin Ⅵ and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis  

作　　者：Fangping Chen Qing Liang Lijie Mao Yanrong Yin b Lixin Zhang Cuidi Li Changsheng Liu 

机构地区：[1]Key Laboratory for Ultrafine Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,PR China [2]Engineering Research Center for Biomedical Materials of Ministry of Education,East China University of Science and Technology,Shanghai,200237,PR China [3]Department of Orthopaedics,Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases,Shanghai Institute of Traumatology and Orthopaedics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,PR China [4]Frontiers Science Center for Materiobiology and Dynamic Chemistry,East China University of Science and Technology,Shanghai,200237,PR China

出　　处：《Bioactive Materials》2022年第4期335-344,共10页生物活性材料（英文）

基　　金：funded by National Key Research and Development Program of China(No.2016YFC1102900);National Natural Science Foundation of China(No.51772100 and No.32171342);Shanghai Science and Technology Agriculture Project(No.202002080002F01474);Shanghai Pujiang Program(16PJD015);Joint Fund for equipment pre-research of the ministry of education(6141A02022618).

摘　　要：Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function.Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis.In this study,we first systematically investigated the roles of Chinese medicine Asperosaponin Ⅵ(ASP Ⅵ)on osteogenic mineralization of BMSCs and osteoclastogenesis of BMMs,and then explored the synergistic effect of ASP Ⅵ and BS(BMP-2 immobilized in 2-N,6-O-sulfated chitosan)on bone formation.The result showed that ASP Ⅵ with the concentration lower than 10^(-4) M contributed to the expression of osteogenic gene and inhibited osteoclastic genes RANKL of BMSCs.Simultaneously,ASP Ⅵ significantly reduced the differentiation of mononuclear osteoclasts in the process of osteoclast formation induced by M-CSF and RANKL.Furthermore,by stimulating the SMADs,TGF-β1,VEGFA,and OPG/RANKL signaling pathways,ASBS(ASP Ⅵ and BS)substantially enhanced osteogenesis,greatly promoted angiogenesis,and suppressed osteoclastogenesis.The findings provide a new perspective on osteoporosis care and prevention.

关 键 词：AsperosaponinⅥ RHBMP-2 OSTEOPOROSIS OSTEOGENESIS OSTEOCLASTOGENESIS 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]