大豆油-月桂酸结构脂质对C57BL/6J小鼠脂质代谢的影响  

Effects of Soybean Oil Based Structured Lipids Containing Lauric Acid on Lipid Metabolism in C57BL/6J Mice

作　　者：王志高 吴莹 王博 袁建 鞠兴荣 何荣 Wang Zhigao;Wu Ying;Wang Bo;Yuan Jian;Ju Xingrong;He Rong(College of Food Science and Engineering,Collaborative Innovation Center for Modern Grain Circulation and Safety,Key Laboratory of Grains and Oils Quality Control and Processing,Nanjing University of Finance and Economics,Nanjing 210023)

机构地区：[1]南京财经大学食品科学与工程学院江苏省现代粮食流通与安全协同创新中心江苏高校粮油质量安全控制及深加工重点实验室,南京210023

出　　处：《中国食品学报》2022年第4期83-94,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(32101872,31871750);江苏省自然科学基金项目(BK20200837);江苏省农业自主创新项目(CX(21)3041)。

摘　　要：大豆油-月桂酸新型结构脂质的开发及其对脂质代谢影响的研究,为开发控制肥胖及相关疾病的结构脂质提供理论依据。以大豆油和月桂酸为原料,通过酶促酯交换反应合成MLM(中长链)型结构脂质。选取20只健康的C57BL/6J型实验小鼠均分为4组,喂食不同的饲料,分别是标准饮食组(普通饲料+生理盐水组),普通饲料+大豆油组,普通饲料+结构脂质组,高脂饲料+结构脂质组。喂食30 d后观察小鼠体重的变化,并检测小鼠血清中甘油三酯(TG)、总胆固醇(TC)、高密度胆固醇(HDL-C)、低密度胆固醇(LDL-C)和脏器指数等相关指标,分析大豆油-月桂酸新型结构脂质改善脂质代谢抑制肥胖的效果。研究表明,添加大豆油-月桂酸结构脂质会影响接受标准饮食或高脂饮食小鼠的吸收,从而有效防止小鼠体重增加。小鼠血清中的TC、TG、HDL-C和LDL-C水平得到有效调节,而丙氨酸氨基转移酶和天冬氨酸转氨酶的活性无明显变化。表明获得的新型结构脂质具有控制肥胖的良好潜力,且不会产生副作用。The aim of this study was to prepare a new kind of structured lipids consisting of soybean oil and lauric acid aimed at studying their effects on lipid metabolism and obesity inhibition of C57 BL/6 J mice, thus providing a theoretical basis for the development of structured lipids that can effectively control obesity and reduce related diseases. The MLMtype(medium-long-medium chain) SLs were synthesized by enzymatic interesterification from soybean oil and lauric acid.Twenty healthy C57 BL/6 J mice were selected and divided into four groups by feeding different feeds, ordinary feed +normal saline group(DF + NS), high-fat feed + structural lipid group(HF + LaSLs), ordinary feed + soybean oil group(DF + SO), and ordinary feed + structural lipid group(DF + LaSLs). The changes in body weight of mice after feeding for 30 days were observed. Some values including serum triglycerides, cholesterol, high-density cholesterol, low-density cholesterol and organ index were detected to analyze and evaluate the influence of soybean oil lauric acid SLs on improving lipid metabolism and preventing obesity. Studies showed that the addition of soybean oil-lauric acid SLs can affect the absorption of mice receiving a standard diet or a high-fat diet, thereby effectively preventing the weight gain of mice. The levels of triglycerides, total cholesterol, HDL-C and LDL-C in the serum were effectively regulated, and there were no significant change in the activity of aminotransferase(ALT) and aspart Aminotransferase(AST). Additionally, the results indicated that the new structured lipids obtained had good potential for controlling obesity and would not produce side effects.

关 键 词：结构脂质 大豆油 月桂酸 脂质代谢 体重控制 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]