基于高通量测序技术分析恩施州淡豆豉的细菌多样性  

Analysis of Bacterial Diversity in Sojae Semen Praeparatum from Enshi by High-throughput Sequencing

作　　者：马上超 程超 田成 陈业 莫开菊 汪兴平[1,2] MA Shangchao;CHENG Chao;TIAN Cheng;CHEN Ye;MO Kaiju;WANG Xingping(College of Biological Science and Technology,Hubei Minzu University,Enshi 445000,China;Key Laboratory of Biological Resources Protection and Utilization of Hubei Province(Hubei Minzu University),Enshi 445000,China)

机构地区：[1]湖北民族大学生物科学与技术学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室(湖北民族大学),湖北恩施445000

出　　处：《食品科学》2022年第10期156-161,共6页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2018YFD0400104)。

摘　　要：以恩施州不同产地5种细菌型淡豆豉作为实验对象,利用Illumina PE250高通量测序技术,对5种淡豆豉的细菌群落结构及多样性进行研究。结果表明:5种恩施州豆豉的微生物组成多样,共鉴定出17门、24纲、43目、78科、152属以及93种。其中核心优势细菌门为厚壁菌门(Firmicutes),其次为变形菌门(Proteobacteria)、蓝细菌门(Cyanobacteria)和放线菌门(Actinobacteria),优势菌属为芽孢杆菌属(Bacillus)的嗜热淀粉芽孢杆菌(Bacillus thermoamylovorans)和史密斯芽孢杆菌(Bacillus smithii)。各样品间,优势菌属相同,但相对丰度差异较大,其余次要菌属的种类和相对丰度均存在较大差异,显示出恩施淡豆豉菌群结构的多样性。In this study,the bacterial community structure and diversity in five samples of bacteria-fermented Sojae Semen Praeparatum produced in different areas of Enshi Prefecture were investigated using high-throughput sequencing on the Illumina PE250 platform.The results showed that the microbial composition of Sojae Semen Praeparatum was diverse.A total of 17 phyla,24 classes,43 orders,78 families,152 genera and 93 species were identified.Among them,the core dominant phylum were Firmicutes,followed by Proteobacteria,Cyanobacteria and Actinobacteria.The dominant genera were Bacillus thermoamylovorans and Bacillus smithii.The composition of dominant genera in the five samples was the same,but the relative abundance was quite different.There was a significant difference in the composition and relative abundance of the other minor genera.

关 键 词：细菌型 淡豆豉 Illumina PE250 多样性 群落结构 

分 类 号：TS201.3[轻工技术与工程—食品科学]