基于乳清蛋白的骆驼乳中掺假牛乳的检测及热处理对方法的影响  

A Method for Detection of Cow Milk in Adulterated Camel Milk Based on Whey Protein and Effect of Heat Treatment on It

作　　者：李玲玉 王俊 李敏婧 杨迎春[1] 苗静 赵仲凯 杨洁[1] LI Lingyu;WANG Jun;LI Minjing;YANG Yingchun;MIAO Jing;ZHAO Zhongkai;YANG Jie(College of Life Science and Technology,Xinjiang University,Ürümqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《食品科学》2022年第10期329-335,共7页Food Science

基　　金：新疆维吾尔自治区重点研发专项(2018B01003);国家自然科学基金地区科学基金项目(31471645);“十三五”国家重点研发计划重点专项(2019YFC1606103)。

摘　　要：利用超高效液相色谱(ultra-high performance liquid chromatography,UPLC)技术,建立一种以牛β-乳球蛋白为掺假标识物的检测方法,用于定性定量检测骆驼乳中掺假的牛乳,并且探讨不同热处理方式对掺假标识物的影响,以期满足不同商品化驼乳制品的检测需求。结果表明:该方法能有效地检测鲜驼乳、巴氏杀菌驼乳以及驼乳粉中掺假的牛乳,3种类型掺假乳样本的定量检测回归方程线性良好,线性相关系数(R^(2))分别为0.9979、0.9969和0.9978;鲜驼乳、巴氏杀菌驼乳和驼乳粉中掺假牛乳的检出限分别为2%、3%和5%,可满足检测需求;利用该方法在10种不同品牌市售纯驼乳粉中检测出4种掺假驼乳粉产品。UPLC法可以有效地检测骆驼乳及其制品中掺假的牛乳,为骆驼乳行业的掺假检测提供一定的技术方法支持。A method using bovineβ-lactoglobulin as the adulteration marker was developed for the qualitative and quantitative detection of cow milk in adulterated camel milk by ultra-high performance liquid chromatography(UPLC).The effects of different heat treatments on the adulteration marker were discussed to meet the need for the detection of different commercial camel dairy products.The results showed that this method could effectively detect milk in adulterated raw camel milk,pasteurized camel milk and camel milk powder.The regression equations of the three adulterated milk samples showed good linearity,with correlation coefficients(R^(2))of 0.9979,0.9969 and 0.9978,respectively.The limits of detection for the adulterated samples were 2%,3%and 5%,respectively,which could meet the detection requirements.Using this method,out of 10 different brands of commercial pure camel milk powder,four were found to be adulterated.

关 键 词：驼乳 牛乳 掺假 Β-乳球蛋白 超高效液相色谱法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]