刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的研究  

Rosa roxburghii Fruit Wine Improves Glucose and Lipid Metabolism Disorder in Type 2 Diabetic Rats

作　　者：安玉红 周靖 朱德星 AN Yuhong;ZHOU Jing;ZHU Dexing(Guizhou Vocational College of Food Engineering,Guiyang 551499,China)

机构地区：[1]贵州食品工程职业学院,贵州贵阳551499

出　　处：《食品工业科技》2022年第12期361-368,共8页Science and Technology of Food Industry

基　　金：毕节市科技项目(毕科联合字ZY[2017]01号);贵州省林业局科技项目(黔林科合[2020]2011)。

摘　　要：为探究刺梨果酒对2型糖尿病大鼠糖脂代谢紊乱的影响及可能机制。采用高脂高糖膳食联合腹腔注射链佐霉素(Streptozocin,STZ)建立2型糖尿病大鼠模型,将造模成功的大鼠分为刺梨果酒高(8 mL/kg)、中(4 mL/kg)、低(2 mL/kg)剂量组和模型组,并设空白对照组,给药期间每2周测一次空腹血糖,实验时间28 d。实验结束后测量血清和肝脏中高密度脂蛋白胆固醇(High-density lipoprotein cholesterol,HDL-C)、果糖胺(fructosamine,FMN)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low-density lipoprotein cholesterol,LDL-C)、总胆固醇(Total cholesterol,TC)、肝糖原等含量;采用实时灾光定量PCR(real time polymerase chain reaction,RT-PCR)测定肝脏中腺苷酸活化蛋白激酶(AMP-activated protein kinase α,AMPK)、乙酰辅酶A羧化酶(Acetyl-CoA carboxylases alpha,ACACA)、β-羟-β-甲戊二酸单酰辅酶A还原酶(3-hydroxy-3-methylglutaryl coenzyme A reductase, HMG-CoA)、脂肪酸合成酶(fatty acid synthetase, FASN)、和葡萄糖转运载体2(Glucose Transporter 2,GLUT2)、胆固醇7α-羟化酶(Cholesterol 7α-hydroxylase,CYP7A1)等mRNA相对表达量。结果表明,与模型组相比,刺梨果酒可减缓2型糖尿病大鼠体重减轻和多饮、多食的症状;高、中剂量刺梨果酒降低实验大鼠空腹血糖和果糖胺的效果显著(P<0.05);各剂量组均有降低实验大鼠血清和肝脏TC、TG、LDL-C的含量和升高HDL-C含量的作用,其中高、中剂量效果显著(P<0.05);低、中、高刺梨果酒均可显著(P<0.05)上调AMPK、GLUT2和ACACA mRNA表达量,低、中、高刺梨果酒均可上调FASN mRNA表达量,其中高、中剂量组上调FASN mRNA表达量显著(P<0.05);中、高剂量刺梨果酒可显著(P<0.05)下调G6Pase、PEPCK、HMG-COA和CYP7A1 mRNA表达量。结论:刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的机制可能与通过抑制内源性胆固醇、增加脂肪的从头合成及提高葡萄糖跨膜转速率有关。This study aimed to investigate the effect of Rosa roxburghii fruit wine on glucose and lipid metabolism disorder in type 2 diabetic rats and its possible mechanisms. The model of type 2 diabetic mice was established by high fat and high sugar diet combined with intraperitoneal injection of streptozocin(STZ). The rats were divided into high(8 mL/kg),medium(4 mL/kg), low(2 mL/kg) dose groups and model group, blank group. Fasting blood glucose was measured every two weeks for 28 d. After the experiment, the contents of High-density lipoprotein cholesterol(HDL-C), fructosamine(FMN), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), total cholesterol(TC) and hepatic glycogen were measured in serum and liver. The relative mRNA expressions of AMP-activated protein kinase α(AMPK), Acetyl-CoA carboxylases alpha(ACACA), 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMG-CoA), fatty acid synthase(FASN), Glucose Transporter 2(GLUT2) and Cholesterol 7α-hydroxylase(CYP7A1) in liver were measured by real time polymerase chain reaction(RT-PCR). The results showed that compared with the model group, Rosa roxburghii fruit wine alleviated the symptoms of weight loss, polydipsia and polyphagia in type 2 diabetic rats. High and medium dose Rosa roxburghii fruit wine significantly reduced fasting blood glucose and fructosamine content in experimental rats(P<0.05).The contents of TC, TG and LDL-C in plasma and liver were decreased and the content of HDL-C was increased in each dose group. Among them, the high and medium dose showed significant effects(P<0.05). Each dose group significantly increased the relative expression of AMPK, GLUT2 and ACACA mRNA(P<0.05). High, medium and low dose increased the relative expression of FASN mRNA, and the high and medium dose had significant effects(P<0.05). High and medium dose also significantly decreased the relative expression of G6Pase, PEPCK, HMG-COA and CYP7A1 mRNA(P<0.05).Conclusion: The mechanisms of Rosa roxburghii fruit wine to improve glucose and lipid metabolism disorder

关 键 词：刺梨 刺梨果酒 2型糖尿病 糖代谢 脂代谢 

分 类 号：R363[医药卫生—病理学]