粮食加工品和肉制品中克罗诺杆菌的污染状况及致病性阪崎克罗诺杆菌生物膜形成能力研究  

Study on contamination of Cronobacter spp. and biofilm formation ability of pathogenic Cronobacter sakazakii in cereal processed products and meat products

作　　者：王敏 刘长虹 陈颖[2] 曲天铭 张九凯[2] 王娉[2] WANG Min;LIU Chang-Hong;CHEN Ying;QU Tian-Ming;ZHANG Jiu-Kai;WANG Ping(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China;Chinese Academy of Inspection and Quarantine,Beijing 100176,China;College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]合肥工业大学食品与生物工程学院,合肥230601 [2]中国检验检疫科学研究院,北京100176 [3]吉林农业大学食品科学与工程学院,长春130118

出　　处：《食品安全质量检测学报》2022年第9期2995-3003,共9页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2018YFC1603504)。

摘　　要：目的 了解我国粮食加工品和肉制品中克罗诺杆菌(Cronobacter spp.)的污染情况及致病性。方法对我国8个省份的226份粮食加工品和肉制品的克罗诺杆菌污染情况进行检测,对分离菌株进行种间鉴定,并针对阪崎克罗诺杆菌进行脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分型及生物膜形成能力研究。结果 粮食加工品和肉制品中克罗诺杆菌的检出率分别为21.92%和12.50%。种间鉴定显示,115株分离菌株中有94株阪崎克罗诺杆菌。PFGE和结晶紫染色结果表明,94株阪崎克罗诺杆菌可被分为49个型别,均在36~60 h内达到最大菌膜生产量。结论 上述8个省份粮食加工品和肉制品中存在克罗诺杆菌污染,其中阪崎克罗诺杆菌有多种PFGE带型,且均具有生物膜形成能力,本研究可为食品安全风险评估及标准制定提供基础数据。Objective To investigate the pollution and pathogenicity of Cronobacter spp. in cereal processed products and meat products in China. Methods The contamination of Cronobacter spp. in 226 cereal processed samples and meat samples from 8 provinces were detected, the isolated Cronobacter spp. strains was identified among species, and pulsed field gel electrophoresis(PFGE) was used to classify the molecules typing of Cronobacter sakazakii as well as to study the ability of biofilm formation. Results The detection rates of Cronobacter spp. in cereal processed samples and meat samples were 21.92% and 12.50%, respectively. Species identification showed that 94 Cronobacter sakazakii strains were isolated from 115 isolates. The results of PFGE and crystal violet staining showed that 94Cronobacter sakazakii strains could be divided into 49 types and reached the maximum membrane production within 36-60 h. Conclusion Cronobacter spp. is widely found in cereal processed products and meat products in the 8provinces mentioned above, among them, Cronobacter sakazakii has a variety of PFGE types and biofilm formation ability, and this study could provide basic data for food safety risk assessment and standard formulation.

关 键 词：克罗诺杆菌 分子分型 生物膜形成 种间鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]