PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons  

作　　者：Wei-Tao Yan Wen-Juan Zhao Xi-Min Hu Xiao-Xia Ban Wen-Ya Ning Hao Wan Qi Zhang Kun Xiong 

机构地区：[1]Department of Human Anatomy and Neurobiology,School of Basic Medical Science,Central South University,Changsha,Hunan Province,China [2]Department of Dermatology,Xiangya Hospital,Central South University,Changsha,Hunan Province,China [3]Department of Human Resources,Third Xiangya Hospital of Central South University,Changsha,Hunan Province,China [4]Hunan Key Laboratory of Ophthalmology,Changsha,Hunan Province,China [5]Key Laboratory of Emergency and Trauma,Ministry of Education,College of Emergency and Trauma,Hainan Medical University,Haikou,Hainan Province,China

出　　处：《Neural Regeneration Research》2023年第2期357-363,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.81772134,81971891,82172196,81571939(ail to KX);the Key Laboratory of Emergency and Trauma(Hainan Medical University)of Ministry of Education,No.KLET-202108(to KX);the Fundamental Research Funds for the Central Universities of Central South University of China,No.2020zzts218(to WTY);Hunan Provincial Innovation Foundation for Postgraduate of China,No.CX20200116(to WTY)。

摘　　要：PANoptosis is a newly identified type of regulated cell death that consists of pyroptosis,apoptosis,and nec roptosis,which simultaneously occur during the pathophysiological process of infectious and inflammatory diseases.Although our previous lite rature mining study suggested that PANoptosis might occur in neuronal ischemia/repe rfusion injury,little experimental research has been reported on the existence of PANoptosis.In this study,we used in vivo and in vitro retinal neuronal models of ischemia/repe rfusion injury to investigate whether PAN optosis-like cell death(simultaneous occurrence of pyroptosis,apo ptosis,and necroptosis)exists in retinal neuronal ischemia/repe rfusion injury.Our results showed that ischemia/repe rfusion injury induced changes in morphological features and protein levels that indicate PANoptosis-like cell death in retinal neurons both in vitro and in vivo.Ischemia/repe rfusion inju ry also significantly upregulated caspase-1,caspase-8,and NLRP3 expression,which are important components of the PANoptosome.These results indicate the existence of PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons and provide preliminary experimental evidence for future study of this new type of regulated cell death.

关 键 词：apoptosis gasdermin-D(GSDMD) ISCHEMIA/REPERFUSION mixed lineage kinase domain-like protein(MLKL) NECROPTOSIS NOD-like receptor protein 3(NLRP3) PANoptosis PYROPTOSIS receptor-interacting protein kinase 3(RIPK3) retinal neuron 

分 类 号：R774.13[医药卫生—眼科]