高效液相色谱法测定植物油料油脂中生育酚含量  

Determination of tocopherols in oilseeds by reversed phase high performance liquid chromatography

作　　者：张瑜 王雪妍 汪雪芳[1] 戚欣 马飞[1] 喻理[1] 张良晓[1,2] 李培武 ZHANG Yu;WANG Xueyan;WANG Xuefang;QI Xin;MA Fei;YU Li;ZHANG Liangxiao;LI Peiwu(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Quality Inspection and Test Center for Oilseed Products,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Hubei Hongshan Laboratory,Wuhan 430070,China)

机构地区：[1]中国农业科学院油料作物研究所,农业农村部油料作物生物学与遗传育种重点实验室,农业农村部油料及制品质量监督检验测试中心,农业农村部油料产品质量安全风险评估实验室(武汉),湖北武汉430062 [2]湖北省洪山实验室,湖北武汉430070

出　　处：《食品与发酵工业》2022年第12期243-248,共6页Food and Fermentation Industries

基　　金：国家重点研发计划“乡村产业共性关键技术研发与集成应用”重点专项(2021YFD1600101);国家自然科学基金委面上项目(31871886),财政部和农业农村部:国家现代农业产业技术体系(CARS-12)。

摘　　要：该研究建立了油料油脂中生育酚含量高效液相色谱法测定方法。样品加入异丙醇和0.1 g抗氧化剂2,6-二叔丁基对甲酚,经涡旋和超声提取,过0.22μm的有机滤膜,采用Poroshell 120 SB-C_(18)液相色谱柱分离,以90%(体积分数)甲醇-水为流动相、流速0.75 mL/min,柱温30℃,紫外检测波长294 nm,外标法定量分析。方法学考察结果显示,该方法10 min内可以实现4种生育酚基线分离。α-生育酚、β-生育酚、γ-生育酚、δ-生育酚的检出限分别是0.16、0.21、0.20、0.22μg/g,定量限分别是0.53、0.68、0.66、0.72μg/g,相关系数均大于0.9992,且回收率为87%~109%,精密度为1.88%~9.89%。该方法提取过程简单且提取效率高,检测时间短,灵敏度高,重复性好,可满足实际油料样品中生育酚含量的检测需要。In this study,analytical method of four tocopherols in oilseeds and edible oils was developed by reversed-phase high performance liquid chromatography.The sample was firstly dissolved by isopropanol and 0.1 g antioxidant(BHT)was added,then the mixture was extracted by vortex,ultrasonication and centrifugation.Finally,the mixture passed through 0.22μm organic filter membrane for the purification.Poroshell 120 SB-C_(18) liquid chromatographic column was used with 90%methanol-water as mobile phase for the detection of tocopherols.The validation results indicated that the complete separation of four tocopherols could be achieved within 10 min by quantitative analysis on a high performance liquid chromatography with a flow rate of 0.75 mL/min,a column temperature of 30℃and a detection wavelength of 294 nm.The recoveries of the four tocopherols were in the range of 87%-109%,and the precision(RSD)was in the range of 1.88%-9.89%.The limits of detection(LODs)forα-tocopherol,β-tocopherol,γ-tocopherol andδ-tocopherol were 0.16,0.21,0.20 and 0.22μg/g,and the limits of quantification(LOQs)were 0.53,0.68,0.66 and 0.72μg/g respectively.The correlation coefficients were higher than 0.9992.This method has a simple extraction process,high extraction efficiency and sensitivity,which meets the actual sample detection requirements.

关 键 词：生育酚 高效液相色谱 检测 植物油料 食用植物油 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]