分子印迹技术在食品腐败菌与致病微生物检测中的应用  

Molecular Imprinting Technology in Detection of Food Spoilage Bacterial and Pathogenic Microorganism

作　　者：任涛涛 陈金嫒 靳雨婷 汤轶伟 王向红[2] 王硕 Ren Taotao;Chen Jin′ai;Jin Yuting;Tang Yiwei;Wang Xianghong;Wang Shuo(College of Food Science&Project Engineering,Bohai University,Jinzhou 121013,Liaoning;College of Food Science and Technology,Hebei Agricultural University,Baoding 071001,Hebei;Tianjin Key Laboratory of Food Science and Health,School of Medicine,Nankai University,Tianjin 300071)

机构地区：[1]渤海大学食品科学与工程学院,辽宁锦州121013 [2]河北农业大学食品科技学院,河北保定071001 [3]天津市食品科学与健康重点实验室,南开大学医学院,天津300071

出　　处：《中国食品学报》2022年第6期435-444,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2017YFF0211300)。

摘　　要：由微生物引起的食品污染和腐败是全球食品工业面临的两个主要问题,也是各国政府对食品质量安全与食品供应保障的关注点。准确、快速监测食品中微生物的污染状况,对降低食物浪费和食源性疾病爆发具有重要意义。分子印迹聚合物具有易制备、高特异性、性能稳定等特点,被广泛用于食品腐败菌与致病微生物的检测。本文综述微生物分子印迹聚合物的合成方法,包括乳液聚合法、电化学聚合法和微接触印迹法;介绍基于分子印迹聚合物的荧光、电化学传感器、表面等离子共振、石英晶体微天平、共振光散射等方法在食品腐败菌与致病微生物检测中的应用,并对该技术的应用前景与发展方向进行展望。Food pollution and corruption caused by microorganisms are two major problems facing the global food industry,and also the focus of governments on food quality and safety and food supply security.Accurate and rapid monitoring of microbial contamination in food is of great significance to reduce food waste and foodborne disease outbreaks.Molecularly imprinted polymers(MIPs)have been widely used in the detection of food spoilage bacteria and pathogenic microorganisms due to their easy preparation,high specificity and stable performance.In this paper,the synthetic methods of microbial molecularly imprinted polymers,including emulsion polymerization,electrochemical polymerization and microcontact imprinting method,are reviewed.The research achievements of MIPs-based detection methods,such as fluorescence,electrochemical sensors,surface plasmon resonance,quartz crystal microbalance,and resonance light scattering,are introduced in the detection of food spoilage bacteria and pathogenic microorganisms.The application prospect and development direction of this technology are also prospected.

关 键 词：分子印迹技术 食品腐败菌 致病微生物 检测方法 

分 类 号：TS207.4[轻工技术与工程—食品科学] O631.3[轻工技术与工程—食品科学与工程]