沙门氏菌检验用培养基的质量控制标准研究  

Study on the quality control standard of culture medium for Salmonella

作　　者：陈怡文 张晓东 余文[1] 任秀[1] 刘娜[1] 崔生辉[1] CHEN Yiwen;ZHANG Xiaodong;YU Wen;REN Xiu;LIU Na;CUI Shenghui(National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国食品卫生杂志》2022年第3期491-497,共7页Chinese Journal of Food Hygiene

基　　金：科技部“食品安全关键技术研发”重点专项(2017YFC1601400)。

摘　　要：目的通过筛选沙门氏菌检验用培养基的质控菌株,为GB 4789.28—2013标准的修订和企业培养基的质控提供依据。方法使用GB 4789.28中规定的菌株及从食品中分离的9株沙门氏菌,分别为纽波特沙门氏菌、婴儿沙门氏菌、猪霍乱沙门氏菌、布里丹沙门氏菌、科特布斯沙门氏菌、亚利桑那肠沙门氏菌、都柏林沙门氏菌、阿贡纳沙门氏菌和鸭沙门氏菌,通过定量和半定量方法测试10种不同品牌的RV肉汤、亚硒酸盐胱氨酸增菌液、四硫磺酸钠亮绿培养基和亚硫酸铋琼脂、HE琼脂、木糖赖氨酸脱氧胆盐分离培养基的生长率和选择性的差异,以评价市场现有培养基的质量差异及GB 4789.28—2013中所用的质控菌株是否能满足沙门氏菌培养基的质量控制。结果结果显示用同一株菌进行检测时,不同品牌的培养基的生长率和选择性存在差异,且猪霍乱沙门氏菌(Salmonella choleraesuis)和亚利桑那肠沙门氏菌(Salmonella arizona)对培养基的质量有更高的要求。结论建议培养基生产企业及微生物实验室在培养基检验过程中,使用从食品中分离出的,对培养基质量要求高的质控菌株,同时质控方法应予以高度规范化,以保障检验数据稳定、可靠。Objective The purpose of this study was to provide basis for the revision of GB 4789.28—2013 and the quality control of culture media by screening the quality control strains of culture media for salmonella test.Methods The strains specified in GB 4789.28 and 9 salmonella strains(Salmonella newport,salmonclla Infantis,Salmonella choleraesuis,Salmonella blegdam,Salmonella kottbus,Salmonella arizona,Salmonella dublin,Salmonella agona,Salmonella.anatis)isolated from real food were used to test the growth rate and selectivity of 10 different brands of media including Rappaport Vassiliadis Salmonella Enrichment,Selenite Cystine Broth,Tetrathionate Broth Base enriched broth,Bismuth Sulfite Agar,Hektoen Enteric Agar,Xylose Lysine Desoxycholate Agar by quantitative and semi-quantitative methods.It was evaluated whether the quality control strain used in GB4789.28—2013 could meet the quality control of salmonella media.Results The results showed that the growth rate and selectivity of different media brands were different when the same strain was used,and Salmonella choleraesuis and Salmonella arizonae had higher requirements on the quality of media.Conclusion It is suggested that medium production enterprises and microbiology laboratories should use strains isolated from food with higher quality requirement in medium testing,and the quality control methods should be highly standardized to ensure the stability and reliability of test data.

关 键 词：培养基 质量控制 沙门氏菌 食品 

分 类 号：R155[医药卫生—营养与食品卫生学]