流式细胞仪在环境微生物学实验教学中的应用与实践  

Application and Practice of Flow Cytometer in Experimental Teaching of Environmental Microbiology

作　　者：翟利芳[1] 展思辉[1] 王忠[1] ZHAI Lifang;ZHAN Sihui;WANG Zhong(College of Environmental Science&Engineering,Nankai University,Tianjin 300350,China)

机构地区：[1]南开大学环境科学与工程学院,天津300350

出　　处：《实验室研究与探索》2022年第4期183-185,210,共4页Research and Exploration In Laboratory

基　　金：天津市自然科学重点基金项目(18JCZDJC39700)。

摘　　要：根据环境微生物学实验课程的教学安排,将流式细胞仪实验技术融入实验课程,设计了“抗生素对铜绿微囊藻生长性能的影响”示例实验,实现环境微生物学、现代仪器分析技术与学生实际操作能力培养的有效融合。实践表明,实验教学中融入新技术方法,与传统测试方法进行对比,既丰富实验教学内容又增加实验趣味性,实验教学效果得到显著提升,同时大大提高了大型仪器在实验教学中的应用频率。According to the teaching arrangement of the environmental microbiology experiment course,the flow cytometer experiment technology is integrated into the experiment course.A demonstration experiment of“Effect of antibiotics on the growth performance of microcystis aeruginosa”is designed.This realizes the effective integration of environmental microbiology,instrumental analysis technologyand the cultivation of students’hands-on ability.The new technology of flow cytometer is integrated into the experimental teaching compared with the traditional test method.As a result,the content of experiment teaching is colorful and interesting,which greatly improves the effect of experiment teaching,and the application frequency of large instruments in experimentteaching has also been significantly improved.

关 键 词：流式细胞仪 抗生素 微藻 实验教学 

分 类 号：X172[环境科学与工程—环境科学]