野梧桐种子总酚含量及其生物活性的研究  

Study on total phenolic contents and biological activities of Mallotus japonicus seeds

作　　者：顾雯艳 陈佳[1] 张小惠[1] 张玉倩 邵建华[1] 赵春超[1] GU Wenyan;CHEN Jia;ZHANG Xiaohui;ZHANG Yuqian;SHAO Jianhua;ZHAO Chunchao(College of Biological Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2022年第3期97-101,116,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31201563);贵州大学绿色农药与生物工程教育部重点实验室开放课题(2021GDGP0103/2021GDGP0104)。

摘　　要：为评价野梧桐种子的生物活性,采用Folin-Ciocalteu比色法测定野梧桐种子石油醚提取物(Ⅰ)和95%乙醇提取物(Ⅱ)及其乙酸乙酯萃取物(Ⅲ)和正丁醇萃取物(Ⅳ)的总酚含量;采用体外活性测试方法,分别对其α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶抑制活性及抗氧化活性(DPPH、ABTS和FRAP法)进行评价。结果表明:乙酸乙酯萃取物总酚含量最高,达到(679.84±14.65)mmol·g^(-1);除石油醚提取物(Ⅰ)外,Ⅱ、Ⅲ和Ⅳ均具有一定的酶抑制活性及抗氧化活性,其中乙酸乙酯萃取物(Ⅲ)活性最为显著,对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的半数抑制浓度(IC_(50))分别为0.106、0.087、0.058 mg·mL^(-1),清除DPPH、ABTS自由基的IC分别为0.047、0.031 mg·mL^(-1),FRAP值为12.118 mmol·g^(-1)。这说明野梧桐种子可应用于Ⅱ型糖尿病及其慢性并发症的防治,酚类成分可能是其主要活性成分。Inhibiting the activities ofα-amylase,α-glucosidase and aldose reductase and suppressing oxidative stress are the an effective means to prevent and treat type 2 diabetes(T2 D)and its related chronic complications.The total phenolic contents of petroleum ether,95%ethanol,ethyl acetate and n-butanol extracts(Ⅰ—Ⅳ)from Mallotus japonicus seeds were determined by Folin-Ciocalteu assay.In addition,theα-amylase,α-glucosidase,aldose reductase inhibitory activities as well as the antioxidant activity(DPPH,ABTS,FRAP)of fractionsⅠ—Ⅳwere evaluated in vitro.The result showed that the ethyl acetate fraction(Ⅲ)possessed the highest total phenolic content of(679.84±14.65)mmol·g^(-1).Moreover,ethyl acetate fraction(Ⅲ)exhibited the strongest inhibitory activities onα-amylase,α-glucosidase and aldose reductase with ICvalues of 0.106,0.087,0.058 mg·mL^(-1),respectively,and the most significant antioxidant activities with ICvalues of 0.047 mg·mL^(-1)(DPPH),0.031 mg·mL^(-1)(ABTS)were observed,while FRAP value was 12.118 mmol·g^(-1).These findings suggested that polyphenols might be the main active constituents of M.japonicas seeds for preventing and treating T2 D and its related chronic complications.

关 键 词：野梧桐 酚类 Α-淀粉酶 Α-葡萄糖苷酶 醛糖还原酶 抗氧化 

分 类 号：R932[医药卫生—生药学]