A Deep Mesencephalic Nucleus Circuit Regulates Licking Behavior  

作　　者：Di Zheng Jja-Yu Fu Meng-Yu Tang iao-Dan Yu Yi Zhu Chen Jie Shen Chun-Yue Li Shi-Ze Xiel Shan Lin Minmin Luo Xiao-Ming Li 

机构地区：[1]Department of Neurobiology and Department of Neurology,of Second Affliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China [2]NHC and CAMS Key Laboratory of Medical Neurobiology,MOE Frontier Science Center for Brain Research and Brainmachine Integration,School of Brain Science and Brian Medicine,Zhejiang University,Hangzhou 310058,China [3]National Institute of Biological Sciences(NIBS),Bejing 102206,China [4]Chinese Institute for Brain Research,Beijing 102206,China [5]School of Life Sciences,Tsinghua University,Bejing 100084,China [6]Center for Brain Science and Brain-Inspired Intelligence,Research Units for Emotion and Emotion Disorders,Chinese Academy of Medical Sciences,China/Guangdong-Hong Kong-Macao Greater Bay Area,Joint Institute for Genetics and Genome Medicine Between Zhejiang University and University of Toronto,Hangzhou 310058.China

出　　处：《Neuroscience Bulletin》2022年第6期565-575,共11页神经科学通报（英文版）

基　　金：supported by the Key-Area Research and Development Program of Guangdong Province(2019B030335001 and 2018B030334001);the Natural Science Foundation of China(31871070 and 82090031);the Key R&D Program of Zhejiang Province(2020C03009);Funda-mental Research Funds for the Central Universities 2021FZZX001-37,the Non-Profit Central Research Institute Fund of the Chinese Academy of Medical Sciences(2019PT310023);and the CAMS Innovation Fund for Medical Sciences(2019-12M-5-057).

摘　　要：Licking behavior is important for water intake.The deep mesencephalic nucleus(DpMe)has been implicated in instinctive behaviors.However,whether the DpMe is involved in licking behavior and the precise neural circuit behind this behavior remains unknown.Here,we found that the activity of the DpMe decreased during water intake.Inhibition of vesicular glutamate transporter 2-positive(VGLUT2+)neurons in the DpMe resulted in increased water intake.Somatostatin-expressing(SST+),but not protein kinase C-expressing(PKC-8+),GABAergic neurons in the central amygdala(CeA)preferentially innervated DpMe VGLUT2+neurons.The SST+neurons in the CeA projecting to the DpMe were activated at the onset of licking behavior.Activation of these CeA SST+GABAergic neurons,but not PKC-8+GABAergic neurons,projecting to the DpMe was sufficient to induce licking behavior and promote water intake.These findings redefine the roles of the DpMe and reveal a novel CeAssT_DpMevcLUT?cireuit that regulaes icking behavior and promotes water intake.

关 键 词：MIDBRAIN AMYGDALA LICKING Neural Circuit OPTOGENETICS 

分 类 号：R741[医药卫生—神经病学与精神病学]