诺丽发酵汁介导肠道菌群缓解小鼠DSS结肠炎  

Fermented Noni Juice Alleviates DSS Colitis in Mice by Mediating Intestinal Microbiota

作　　者：曲泰齐 张家超[1] 汪瑞敏 刘四新[1] 任发政[2] 李从发[1] Qu Taiqi;Zhang Jiachao;Wang Ruimin;Liu Sixin;Ren Fazheng;Li Congfa(College of Food Science and Engineering,Hainan University,Haikou 570228;Department of Nutrition and Health,China Agricultural University,Beijing 100193)

机构地区：[1]海南大学食品科学与工程学院,海口570228 [2]中国农业大学营养与健康系,北京100193

出　　处：《中国食品学报》2022年第8期118-126,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金地区项目(31760456)。

摘　　要：溃疡性结肠炎(UC)发病率在全球范围内不断上升,已成为严重的健康问题。诺丽果浆作为新食品原料含有丰富的活性物质,具有多种对人体有益的保健功能,如抗炎、抑菌和降血脂等。将小鼠分为3组,分别为空白组(C)、模型组(M)和诺丽发酵汁(FNJ)饲养组(N)。M组和N组给予葡聚糖硫酸钠(DSS)灌胃以诱导结肠炎,1周后对N组小鼠进行FNJ灌胃,两周后处死并测量相关指标。结果表明:N组小鼠疾病活动指数明显降低,结肠组织病理学改变明显减少。同时,FNJ处理的小鼠血清促炎细胞因子降至对照组水平。与M组相比,FNJ能调控结肠炎小鼠肠道菌群组成,降低厚壁菌门的相对丰度,并增加拟杆菌门的相对丰度,提示FNJ能介导肠道菌群缓解小鼠DSS结肠炎,研究结果可为开发FNJ的营养功能和药用价值提供科学依据,为维持肠道稳态和缓解结肠炎提供新途径。Ulcerative colitis(UC)incidence is rising globally,and has become a serious health problem in Asia.Noni(Morinda citrifolia L.),a kind of medicinal and edible plant,is rich in active substances.Studies have shown that noni fruit has a variety of beneficial health functions such as anti-inflammatory,antibacterial and lowering blood lipids.In this study,mice were divided into three groups:control group(C),model group(M),and naturally fermented noni juice(FNJ)treated group(N).Groups M and N were gavaged with dextran sulfate sodium(DSS)to induce colitis.One week later,mice in group N were gavaged with FNJ.After two weeks,they were killed and related indicators were measured.The results showed that the disease activity index of mice in group N was significantly reduced,and the histopathological changes of colon were alleviated.Meanwhile,serum pro-inflammatory cytokines in FNJ-treated mice were also modulated to normal levels.Compared with the M group,FNJ can also regulate the composition of the intestinal microbiota of mice with colitis,with the relative abundance of Firmicutes reducing and that of Bacteroides increasing.This research provides a scientific basis for developing the nutritional function and medicinal value of FNJ,and supplies new ideas for the intestinal homeostasis and treatment of colitis.

关 键 词：诺丽发酵汁 肠道菌群 结肠炎 高通量测序 

分 类 号：R574[医药卫生—消化系统] TS201.4[医药卫生—内科学]