Exosome-mediated aptamer S58 reduces fibrosis in a rat glaucoma filtration surgery model  

作　　者：Qian-Yi Lin Xiang-Ji Li Yu Leng Xiao-Min Zhu Min Tang Yi Lin Wang-Du Luo Bing-Cai Jiang Xia Chen Lin Xie 

机构地区：[1]Department of Ophthalmology,The Third Affiliated Hospital of Chongqing Medical University,Chongqing,China [2]Department of Ophthalmology,Daping Hospital of Army Medical University,Chongqing,China

出　　处：《International Journal of Ophthalmology(English edition)》2022年第5期690-700,共11页国际眼科杂志（英文版）

基　　金：National Natural Science Foundation of China (No.81700836,No.81470629,No.81670860);Chongqing Natural Research Foundation (No.cstc 2018jcyj AX0034)。

摘　　要：AIM:To confirm whether exosome-mediated delivery of aptamer S58(Exo-S58) has a better antifibrotic effect than naked S58 in human conjunctival fibroblasts(HCon Fs) and a rat glaucoma filtration surgery(GFS) model.METHODS:To enhance the effective reaction time of aptamer S58 in vivo, we loaded aptamer S58 into exosomes derived from HEK293 T cells by PEI transfection to determine the effect of Exo-S58 in HCon Fs and a rat GFS model.RESULTS:Exo-S58 can significantly reduce cell proliferation, migration and fibrosis in TGF-β2-induced HCon Fs. In an in vivo experiment, Exo-S58 treatment prolonged filtering bleb retention and reduced fibrosis compared with naked S58 treatment in GFS rats.CONCLUSION:The exosomes are safe and valid carriers to deliver aptamers. Furthermore, Exo-S58 exhibited superior antifibrotic effect than naked S58 both in HCon Fs cells and rat GFS models.

关 键 词：NANOMEDICINE EXOSOMES APTAMER drug delivery glaucoma surgery transforming growth factor-beta ANTI-FIBROSIS 

分 类 号：R779.6[医药卫生—眼科]