天门地区鲊广椒中细菌群落结构及乳酸菌类群研究  

Bacterial community structure and lactic acid bacterial taxa in Zha-chili from Tianmen region

作　　者：席啦 向凡舒 张彦[3] 张海波[3] 郭壮[1,2] XI La;XIANG Fanshu;ZHANG Yan;ZHANG Haibo;GUO Zhuang(Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory,Hubei University of Arts and Science,Xiangyang 441053,China;Research Institute of Biotechnology,Angel Yeast Co.Ltd.,Yichang 443003,China)

机构地区：[1]湖北文理学院湖北省食品配料工程技术研究中心,湖北襄阳441053 [2]湖北文理学院乳酸菌生物技术与工程襄阳市重点实验室,湖北襄阳441053 [3]安琪酵母股份有限公司生物技术研究院,湖北宜昌443003

出　　处：《食品与发酵工业》2022年第17期122-128,共7页Food and Fermentation Industries

基　　金：湖北省教育厅科学技术研究重点项目(D20212601);湖北文理学院教师科研能力培育基金项目(2020kypygp010);湖北文理学院大学生创新创业训练计划项目(S202110519019)。

摘　　要：该研究采用MiSeq高通量测序技术和PICRUSt软件分别解析了天门地区鲊广椒的细菌类群结构与功能,并结合纯培养方式分离、鉴定和保藏了其中的乳酸菌。结果显示,15份样品被分为2个聚类,聚类Ⅰ中细菌主要隶属于乳杆菌属Lactobacillus,相对含量为89.77%;聚类Ⅱ中细菌主要隶属于克雷伯氏菌属Klebsiella、小坂菌属Kosakonia和四联球菌属Tetragenococcus,相对含量分别为19.81%、12.24%和10.89%,经Mann-Whitnay检验发现2个聚类在Lactobacillus、Klebsiella和Kosakonia等菌属上存在极显著差异(P<0.001);同时,Lactobacillus与Kosakonia和Pseudomonas等菌属之间还呈现出显著负相关关系(P<0.05)。2个聚类在15个功能上存在显著性差异,但两者均在氨基酸和碳水化合物的转运与代谢功能上具有高表达。乳酸菌分离鉴定结果显示,鲊广椒中蕴含了L.plantarum、L.brevis、L.curvatus和L.sakei等乳杆菌类群,其中L.plantarum为天门地区鲊广椒中的优势乳杆菌。The bacterial community structure and function of Zha-chili from Tianmen region were analyzed by MiSeq high-throughput sequencing and PICRUSt software,respectively,and the lactic acid bacteria were isolated,identified and preserved by a pure culture method.Fifteen samples were divided into two clusters.The results showed that the bacteria in cluster I were mainly affiliated to Lactobacillus,with a relative abundance of 89.77%;the bacteria in cluster II were mainly affiliated to Klebsiella,Kosakonia,and Tetragenococcus,with relative abundances of 19.81%,12.24%,and 10.89%,respectively.The two clusters were found to be highly significantly different(P<0.001)from one another by Mann Whitney test;meanwhile,a significant negative correlation observed between Lactobacillus and other bacterial genera such as Kosakonia and Pseudomonas(P<0.05).There were significant differences in 15 functions among the two clusters,but both of them were highly expressed in the transport and metabolism functions of amino acids and carbohydrates.The results of isolation and identification of lactic acid bacteria showed that L.plantarum,L.brevis,L.curvatus and L.sakei were contained in Zha-chili,and L.plantarum was the dominant Lactobacillus in Zha-chili from the Tianmen region.

关 键 词：MiSeq高通量测序 鲊广椒 细菌多样性 功能预测 乳酸菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]