雨生红球藻蛋白酶解产物的制备和抗氧化活性评价  

Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate

作　　者：何宛诗 许瑾[1] 曹庸[2] 王忠铭[1] 刘晓娟[2] HE Wanshi;XU Jin;CAO Yong;WANG Zhongming;LIU Xiaojuan(Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences,Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development,Guangzhou 510640,China;College of Food Science,South China Agricultural University,Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,Guangzhou 510642,China)

机构地区：[1]中国科学院广州能源研究所,广东省新能源和可再生能源研究开发与应用重点实验室,广东广州510640 [2]华南农业大学食品学院,广东省功能食品活性物重点实验室,广东广州510642

出　　处：《食品工业科技》2022年第17期248-257,共10页Science and Technology of Food Industry

基　　金：广东省新能源和可再生能源研究开发与应用重点实验室开放基金项目(E139kf0301);广东省自然科学基金面上项目(2019A1515011391,2021A1515012158);国家自然科学基金(32172195)。

摘　　要：为了高值化利用藻渣,本文展开雨生红球藻藻渣制备抗氧化蛋白酶解物的研究。采用单因素和响应面优化试验,研究了主要反应条件(底物浓度、加酶量、温度、pH和时间)对酶解产物ABTS自由基清除率和水解度的影响,并对最优酶解物进行抗氧化活性评价及氨基酸分析。结果表明,雨生红球藻蛋白的最佳酶解条件为:底物浓度9%(w:v)、加酶量0.7%(w:w)、温度40℃、pH11.5和时间3 h。最优酶解物的ABTS自由基清除率IC_(50)为481.574μg/mL,FRAP能力为19.641μmol FeSO_(4)·7HO_(2)/g(浓度为1000μg/mL),显著优于蛋白非酶解物。抗氧化酶解物的氨基酸种类丰富,必需氨基酸含量达34.4%,营养价值高,且含较多与抗氧化密切关联的疏水性氨基酸。本研究为雨生红球藻高附加值应用提供了参考。In order to make high value use of the algal residue,this paper presented a study on the preparation of antioxidant enzymatic hydrolysate from Haematococcus pluvialis residue.Single factor investigation and the response surface methodology(RSM) were used to optimize the hydrolysis process of H.pluvialis residue,including substrate concentration,dosage of proteases,dosage of proteases,temperature,pH and time.The resulting hydrolysates were comparatively evaluated for their ABTS scavenging capacity and degree of hydrolysis.Subsequently,the antioxidant ability and amino acid composition of the optimal hydrolysate were investigated.The results showed that under the optimal hydrolysis conditions:Substrate concentration of 9%(w:v),dosage of proteases of 0.7%(w:w),temperature of 40 ℃,pH11.5 and time of 3 h,the highest ABTS scavenging activity with IC_(50)of 481.574 μg/mL and FRAP value of 19.641 μmol FeSO_(4)·7H_(2)O/g(concentration of 1000 μg/mL) were obtained.Antioxidant enzymatic hydrolysate with 34.4% essential amino acid not only contained high nutritional value but also involved many hydrophobic amino acids closely related to antioxidant activity.This research provides a reference for high value-added applications of H.pluvialis.

关 键 词：雨生红球藻 蛋白酶解物 抗氧化活性 响应面试验 氨基酸分析 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]