绿茶多糖提取工艺优化及其抗氧化活性分析  

Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides

作　　者：张轶斌[1] 刘鹏[1] 纪海玉 贾晓昱 田凌 ZHANG Yibin;LIU Peng;JI Haiyu;JIA Xiaoyu;TIAN Ling(College of Biological Engineering,Tianjin Modern Vocational Technology College,Tianjin 300451,China;College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Institute of Agricultural Products Preservation and Processing Technology,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China;Tangshan Shengchuan Agricultural Products Co.,Ltd.,Tangshan 064105,China)

机构地区：[1]天津现代职业技术学院生物工程学院,天津300451 [2]天津科技大学食品工程与生物技术学院,天津300457 [3]天津市农业科学院,农产品保鲜与加工技术研究所,天津300384 [4]唐山市盛川农产品股份有限公司,河北唐山064105

出　　处：《食品工业科技》2022年第20期220-227,共8页Science and Technology of Food Industry

基　　金：天津市科技成果转化项目(21ZYCGSN00060);津南区科技计划项目(20200119);新疆维吾尔自治区乡村振兴产业发展科技行动项目(2021NC040)。

摘　　要：目的:优化绿茶粗多糖的提取工艺,纯化多糖并分析其结构和抗氧化活性。方法:通过单因素实验结合响应面分析的方法,优化提取工艺,采用液相色谱、气相色谱和红外光谱分析多糖结构,并通过体外抗氧化实验研究其抗氧化活性。结果:多糖最佳提取条件为:液料比30:1 mL/g,提取温度60℃,提取时间70 min。此条件下,绿茶多糖的得率可达10.56%。多糖结构分析结果表明,其总糖、蛋白质和糖醛酸的比例分别为90.75%±3.69%、0.92%±0.09%和0.82%±0.07%,分子量约为7.3 kDa,主要由葡萄糖和半乳糖组成(摩尔比1.00:0.13)。体外抗氧化实验结果表明,在2 mg/mL浓度下,GTP对ABTS^(+)自由基、DPPH自由基和羟基自由基(OH·)清除率分别为39.8%、72.2%和32.5%。结论:本工艺中,绿茶多糖得率高,分子量低,且具有较高的抗氧化活性。Objective:To optimize the extraction process of green tea polysaccharide(GTP),purify the polysaccharide and analyze the antioxidant activity in vitro.Methods:The single factor experiments combined with response surface analysis was used to optimize the extraction process of polysaccharides.High performance gel permeation chromatography(HPGPC),gas chromatography(GC)and Fourier transform infrared spectroscopy(FTIR)were used to analyze the preliminary structure of polysaccharides,and the antioxidant activity of polysaccharides was studied by in vitro antioxidant experiments.Results:The optimal extraction conditions for GTP were determined as follows:Liquid/solid ratio 30:1 mL/g,extraction temperature 60℃,extraction time 70 min.Under these conditions,the yield of GTP reached to 10.56%.Structural analysis results showed that the total sugar,protein and uronic acid contents in GTP were 90.75%±3.69%,0.92%±0.09%,and 0.82%±0.07%,respectively,and the average molecular weight was about 7.3 kDa.In vitro antioxidant test results showed that GTP had scavenging rates of 39.8%,72.2% and 32.5% on ABTS^(+)free radical,DPPH free radical and hydroxyl free radical(OH·)at 2 mg/mL,respectively.Conclusion:In this process,green tea polysaccharides had presented high yield,low molecular weight and high antioxidant activity.

关 键 词：绿茶多糖 提取工艺 结构分析 抗氧化活性 

分 类 号：TS201[轻工技术与工程—食品科学]