响应面法优化β-半乳糖苷酶法制备低聚半乳糖工艺  

Optimization of Preparation of Galactooligosaccharides byβ-Galactosidase Using Response Surface Methodology

作　　者：孙聪聪 庞道睿[1] 黎尔纳 李倩[1] 邹宇晓[1] 廖森泰[1] 刘凡[1] SUN Congcong;PANG Daorui;LI Erna;LI Qian;ZOU Yuxiao;LIAO Sentai;LIU Fan(Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Food,Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Products Processing,Guangdong Province,Guangzhou 510600,China;College of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang 212018,China)

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广东广州510600 [2]江苏科技大学生物技术学院,江苏镇江212018

出　　处：《食品工业科技》2022年第22期246-255,共10页Science and Technology of Food Industry

基　　金：广东省重点领域研发计划项目(2019B020213001);广东省农业科学院农业优势产业学科团队建设项目(202119TD);现代农业产业技术体系建设专项(CRAS-18)。

摘　　要：本文以乳糖为起始原料,在单因素实验的基础上,结合响应面分析法考察加酶量、反应温度、反应时间、反应pH等因素对低聚半乳糖总产率和低聚半乳四糖产率的影响,优化β-半乳糖苷酶法制备低聚半乳糖工艺。结果表明,β-半乳糖苷酶法制备低聚半乳糖的最佳工艺参数为起始乳糖浓度300 g/L、加酶量8.25 U/g乳糖、反应温度49℃、反应时间16 h、反应pH5.6。在此条件下,低聚半乳糖总产率为14.61%,低聚半乳四糖产率为3.31%。该方法针对性提高高聚合度低聚半乳糖的产率,可为低聚半乳糖的功能性应用及特医食品的研发提供参考。In this study,lactose was used as raw material,on the basis of single factor experiment and combined with response surface methodology,enzyme addition,reaction temperature,reaction time,reaction pH and other factors were utilized to investigate the influence of the galactooligosaccharides total yield and the galactotetraose yield.The process of enzymatic preparation of galactooligosaccharides byβ-galactosidase was optimized.The results showed that the optimum process parameters for the enzymatic preparation of galactooligosaccharides were as follows:The initial lactose concentration was 300 g/L,the amount of enzyme added was 8.25 U/g lactose,the reaction temperature was 49℃,the reaction time was 16 h,and the reaction pH was 5.6.Under these conditions,the galactooligosaccharides total yield was 14.61%and the galactotetraose yield was 3.31%.The method selectively improved the yield of galactooligosaccharides with high degree of polymerization,which would provide a certain theoretical reference for the functional application of galactooligosaccharides and the further study and development of special medical food.

关 键 词：Β-半乳糖苷酶 低聚半乳糖 制备 优化 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]