优化乳杆菌高产共轭亚油酸的研究进展  

Research Progress on Optimization of Lactobacillus for High Yield of Conjugated Linoleic Acid

作　　者：成晓艳 张丁洁 田艾迪 刘瑛 陈佳璐 陈莹莹 洛雪 Cheng Xiaoyan;Zhang Dingjie;Tian Aidi;Liu Ying;Chen Jialu;Chen Yingying;Luo Xue(College of Food,Shenyang Agricultural University,Shenyang 110866)

机构地区：[1]沈阳农业大学食品学院,沈阳110866

出　　处：《中国食品学报》2022年第10期391-405,共15页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31701623)。

摘　　要：共轭亚油酸(CLA)是亚油酸(LA)的一组位置和空间异构体的总称,其中具有生理活性的异构体是c9,t11-CLA和t10,c12-CLA,它们具有丰富的营养价值和医药价值。在乳球菌属和链球菌属等乳酸菌、丙酸杆菌、瘤胃细菌以及其它菌属中,分离纯化得到的亚油酸异构酶,可催化LA异构化为CLA,利用酶法合成CLA可以有效弥补传统化学合成法的缺陷。本文概述了近年来国内外利用紫外、微波、等离子体诱变、离子注入等物理或化学方法诱变、改造亚油酸异构酶,优化产酶条件和酶学特性,还阐述利用定点突变、基因敲除等基因工程方法,对生产菌株进行改造和培养条件优化,提高CLA产量的相关研究,为进一步筛选出高产共轭亚油酸菌株和CLA的产业化应用提供理论依据。Conjugated linoleic acid is a general term for a group of positional and spatial isomers of linoleic acid, among which the physiologically active isomers are cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid, which has rich nutritional and medicinal values. The isomerase of linoleic acid obtained by isolation and purification in Lactococcus spp. and Streptococcus spp. and other Lactobacillus, Propionibacterium and Rumen bacteria as well as other genera can catalyze the isomerization of linoleic acid to conjugated linoleic acid, and the synthesis of conjugated linoleic acid by enzymatic method can overcome the shortcomings of the traditional chemical synthesis method. This article has reviewed the recent research on the use of UV, microwave, plasma mutagenesis, ion injection and other physical or chemical methods to mutate and modify linoleic acid isomerase, optimize enzyme production conditions and enzymatic properties at home and abroad, and also described the related research on the use of targeted mutation, gene knockout and other genetic engineering methods to genetically modify the production strain and optimize the culture conditions to improve the yield of conjugated linoleic acid, so as to further screen the high yield. This study provides a theoretical basis for further screening of high-yielding conjugated linoleic acid strains and industrial application of conjugated linoleic acid.

关 键 词：共轭亚油酸 亚油酸异构酶 诱变 基因敲除 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]