鱼油对顺铂损伤HEK293细胞及A549细胞的影响  

Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells

作　　者：杜月梅 刘云帆 秦菲[1,2] 高丽萍 DU Yuemei;LIU Yunfan;QIN Fei;GAO Liping(College of Biochemical Engineering,Beijing Union University,Beijing 100023,China;Beijing Municipal Key Laboratory of Biologically Active Substances and Functional Food,Beijing 100191,China)

机构地区：[1]北京联合大学生物化学工程学院,北京100023 [2]生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《食品工业科技》2022年第23期347-352,共6页Science and Technology of Food Industry

基　　金：北京联合大学研究生资助项目;北京联合大学推进产教融合,提升实践应用能力项目。

摘　　要：目的:探讨鱼油对顺铂(cis-dichlorodiamineplatinum(Ⅱ),CDDP)抗A549人肺腺癌细胞活性的影响及对CDDP损伤HEK293细胞的保护作用。方法:体外培养A549和HEK293细胞,将细胞分为对照组、CDDP组、鱼油组、鱼油+CDDP组;分别用不同浓度的鱼油对细胞进行预处理,同时建立CDDP组、鱼油+CDDP组CDDP损伤模型,CCK-8法检测细胞活力,试剂盒测定HEK293细胞中丙二醛(MDA)含量,二还原型谷胱甘肽(GSH)含量,超氧化物歧化酶(SOD)的活力等指标。结果:4~16 mg/L浓度的鱼油能够显著抑制CDDP造成的人胚肾HEK293细胞毒性(P<0.05),其中4 mg/L浓度的鱼油保护效果最佳,且此浓度的鱼油能够显著抑制CDDP(20 mg/L)引起的GSH含量降低以及MDA含量升高(P<0.05)。当鱼油浓度≥32 mg/L时显著增强了CDDP对A549细胞的抑制效果(P<0.05)。结论:一定浓度范围内,鱼油对CDDP致人胚肾细胞HEK293损伤具有一定的保护作用。低浓度鱼油对CDDP所致A549细胞的抑制作用没有明显的影响,高浓度的鱼油增强了CDDP的抗癌效果。Objective:To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum(Ⅱ)(CDDP)against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP.Methods:A549 and HEK293 cells were cultured in vitro,and the cells were divided into control group,CDDP group,fish oil group,fish oil+CDDP group;the cells were pretreated with different concentrations of fish oil,and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time.Cell viability was detected by CCK-8 method,and the kits were used to measure malondialdehyde(MDA)content,direduced glutathione(GSH)content,and superoxide dismutase(SOD)activity in HEK293 cells.Results:Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP(P<0.05).Fish oil at a concentration of 4 mg/L had the best protective effect,and fish oil at this concentration could significantly inhibit CDDP(20 mg/L)decreased GSH content and increased MDA content(P<0.05).When the fish oil concentration was≥32 mg/L,the inhibitory effect of CDDP on A549 cells was significantly enhanced(P<0.05).Conclusion:Within a certain concentration range,fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells.Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells,and high-concentration fish oil enhances the anti-cancer effect of CDDP.

关 键 词：鱼油 顺铂 肾毒性 抗氧化 抗肿瘤 

分 类 号：R151.2[医药卫生—营养与食品卫生学]