铁皮石斛鲜花中多酚氧化酶的酶学特性  

The Enzymatic Properties of Polyphenol Oxidase in Dendrobium officinale Kimura et Migo Flowers

作　　者：夏瑜 张珍林[1,2,3] 闵运江 刘紫薇[1] 耿曾奇 王明晖 XIA Yu;ZHANG Zhenlin;MIN Yunjiang;LIU Ziwei;GENG Zengqi;WANG Minghui(College of Biological and Pharmaceutical Engineering,West Anhui University,Lu'an 237012;Engineering Technology Research Center of Plant Cell Enginering,Lu'an 237012;Anhui Traditional Chinese Medicine Resources Protection and Sustainable Utilization Engineering Laboratory,Lu'an 237012)

机构地区：[1]皖西学院生物与制药工程学院,六安237012 [2]植物细胞工程安徽省工程技术研究中心,六安237012 [3]安徽省中药资源保护与持续利用工程实验室,六安237012

出　　处：《食品工业》2022年第9期117-120,共4页The Food Industry

基　　金：皖西学院2021年度校级科研项目(WXZR202107);皖西学院2021年高层次人才科研启动费资助项目(WGKQ2021030);2021年度高校优秀青年骨干人才国内访学研修项目(gxyq2021209);安徽省自然科学基金(KJ2021A0936)。

摘　　要：以紫外分光光度法探究从铁皮石斛鲜花中提取的多酚氧化酶的酶学性质。结果表明,以邻苯二酚作底物,在460 nm波长条件下,测得铁皮石斛鲜花中提取的多酚氧化酶性质:酶添加量1 mL,酶活性最适pH 6.2,最适底物浓度0.03 mol/L,最适温度30℃,抗坏血酸是试验中抑制效果最佳的抑制剂。The Dendrobium officinale Kimura et Migo flowers were used as raw materials to extract polyphenol oxidase, and the catechol was used as the substrate to make the exploration of its enzymatic properties under the ultraviolet spectrophotometry at 460 nm. According to the results, it was indicated that the optimal condition of enzymatic activity of polyphenol oxidase extracted by using Dendrobium officinale Kimura et Migo flowers was as follows: the addition of 1 mL of enzyme, the optimal pH of 6.2 of enzyme activity. Under 0.03 mol/L of the optimal substrate concentration, the optimum temperature was 30 ℃, and the ascorbic acid was the inhibitor with the best inhibitory effect in this experimental study.

关 键 词：铁皮石斛鲜花 多酚氧化酶 酶学特性 

分 类 号：TS201.25[轻工技术与工程—食品科学]