鳗鱼骨胶原蛋白ACE抑制肽的制备及性质研究  

Preparation and properties of collagen ACE inhibitory peptides from bone of eel(Anguilla japonica)

作　　者：邵燕秋 黄卉[2] 李来好[2] 杨贤庆[2] 陈胜军[2] 郝淑贤[2] 吴燕燕[2] 岑剑伟[2] 邓尚贵 SHAO Yanqiu;HUANG Hui;LI Laihao;YANG Xianqing;CHEN Shengjun;HAO Shuxian;WU Yanyan;CEN Jianwei;DENG Shanggui(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/National Research and Development Center for Aquatic Processing/Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China)

机构地区：[1]浙江海洋大学食品与药学学院,浙江舟山316022 [2]中国水产科学研究院南海水产研究所/国家水产品加工技术研发中心/农业农村部水产品加工重点实验室,广东广州510300

出　　处：《南方水产科学》2022年第6期137-145,共9页South China Fisheries Science

基　　金：国家现代农业产业技术体系资助(CARS-46);“扬帆计划”引进创新创业团队专项(2015YT02H109);广东省重点领域研发计划项目(2019B020225001);中国水产科学研究院基本科研业务费专项资金(2020TD69,2020TD73)。

摘　　要：为研究鳗鱼加工副产物的综合利用,以鳗鱼骨胶原蛋白为原料,采用酶解法制备血管紧张素转化酶(Angiotensinconverting enzyme,ACE)抑制肽。以ACE抑制活性和水解度为评价指标,通过单因素和响应面试验确定最佳的酶解制备条件,并分析酶解产物的分子质量分布和氨基酸组成。结果显示,碱性蛋白酶为最适水解酶,最佳酶解条件为:温度50℃,质量浓度15 g·L^(–1),酶解时间5.25 h,加酶量3.1%(质量分数),pH 9.2。在此条件下,鳗鱼胶原蛋白肽的ACE抑制活性为70.33%,与预测值接近;酶解产物中分子质量小于1 kD的肽占57.02%,1~3 kD的肽占36.55%;氨基酸组成分析表明,酶解产物中与ACE抑制活性有关的疏水性氨基酸(如脯氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸)含量增加。To study the comprehensive utilization of by-products from eel processing,the angiotensin-converting enzyme inhibitory peptide was prepared from eel(Anguilla japonica)bone collagen by enzymatic hydrolysis.The ACE inhibitory activity and degree of hydrolysis were used as evaluation indexes,we determined the optimal hydrolysis conditions by single factor and response surface experiments.Furthermore,the amino acid composition and molecular mass distribution of the hydrolysate prepared under the optimized conditions were determined.The results show that alkaline protease was the optimal enzyme,and the optimal hydrolysis conditions were as follows:temperature of 50℃,mass concentration of 15 g·L^(−1),hydrolysis time of 5.25 h,enzyme dosage of 3.1%(Mass fraction)and pH of 9.2.Under these conditions,the ACE inhibitory activity was 70.33%,which was close to the predicted value.The molecular weight of peptides below 1000 D and 1000–3000 D in enzymatic hydrolysate accounted for 57.02%and 36.55%,respectively.Amino acid composition analysis shows that the content of hydrophobic amino acids related to ACE inhibition activities(Such as Pro、Val、Ile、Leu、Phe)increased.

关 键 词：鳗鱼 骨胶原蛋白 ACE抑制肽 酶解 分子质量分布 氨基酸分析 

分 类 号：TS[轻工技术与工程]