苦荞不同部位酚类化合物组成与抗氧化活性  

Phenolic compounds profile and antioxidant activities of different fractions of Tartary buckwheat

作　　者：陈月 朱勇 秦礼康[1,2] CHEN Yue;ZHU Yong;QIN Li-kang(School of Liquor and Food Engineering,Guizhou University,Guiyang,Guizhou 550025,China;National and Local Joint Engineering Centre for the Development and Utilization Technology of Drug and Food Resources in Southwest China,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学西南药食两用资源开发利用国家地方联合工程研究中心,贵州贵阳550025

出　　处：《食品与机械》2022年第11期15-19,共5页Food and Machinery

基　　金：贵州省科技计划项目(编号:黔科合基础[2019]1071号,黔科合支撑[2020]1Y171号);贵州大学引进人才科研项目(编号:贵大人基合字[2017]46号)。

摘　　要：目的:研究苦荞不同部位酚类化合物组成与抗氧化活性。方法:利用70%乙醇分别提取苦荞壳、苦荞麸皮和苦荞粉三部分中的酚类物质,并分析提取物的酚类化合物组成和抗氧化活性。结果:麸皮部位总酚、总黄酮分别为(3042±31)mg GAE/100 g·DW和(5290±233)mg RE/100 g·DW,分别占全苦荞的60.8%和67.7%。芦丁是麸皮提取物中的主要酚类化合物,其含量为(5152±61)mg/100 g·DW。苦荞壳、苦荞麸皮和苦荞粉提取物均表现出抗氧化活性,其中苦荞麸皮提取物的抗氧化活性最强,其ORAC、FRAP、DPPH自由基清除率和ABTS自由基清除率分别为(1503.3±249.5)μmol/g·DW、(8477.5±441.1)mg TE/100 g·DW、(5642.4±241.3)mg TE/100 g·DW和(16764±742)mg TE/100 g·DW。结论:麸皮是苦荞酚类化合物的主要富集部位,这些化合物表现出较强的抗氧化活性。Objective:This study aimed to investigate the phenolic compounds profile and antioxidant activities of different fractions of Tartary buckwheat.Methods:Tartary buckwheat was ground and sieved into shell,bran and powder.Phenolic compounds in tartary buckwheat were extracted with 70%ethanol,and phenolic compounds composition and antioxidant activity of the extract were analyzed.Results:In Tartary buckwheat bran,the total phenolics and flavonoids were(3042±31)mg GAE/100 g·DW and(5290±233)mg RE/100 g·DW,accounting for 60.8%,67.7%of the total Tartary buckwheat,respectively.Rutin was the dominant phenolic compounds in bran extract,and the content was(5152±61)mg/100 g·DW.The extracts of Tartary buckwheat hull,bran and powder all showed antioxidant activities,among which the bran extracts presented the highest antioxidant activities.The ORAC,FRAP,DPPH and ABTS values were(1503.3±249.5)μmol/g·DW,(8477.5±441.1)mg TE/100 g·DW,(5642.4±241.3)mg TE/100 g·DW and(16764±742)mg TE/100 g·DW,respectively.Conclusion:Bran is the major fraction enriching phenolic compounds which showed elevated antioxidant activities.

关 键 词：苦荞 麸皮 酚类化合物 抗氧化活性 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]