超声提取福鼎白茶总黄酮工艺 优化及抗氧化能力研究  

Study on ultrasonic extraction and antioxidant capacity of total flavonoids from Fuding white tea

作　　者：傅贤明 卢诗[1] 黄欣 陈健敏 FU Xian-ming;LU Shi;HUANG Xin;CHEN Jian-min(School of Pharmacy and Medical Technology,Putian University,Putian 351100,Fujian,China;Key Laboratory of Pharmaceutical Analysis and Laboratory Medicine Fujian Province University,Putian University Putian 351100,Fujian,China)

机构地区：[1]莆田学院药学与医学技术学院,福建莆田351100 [2]莆田学院药物分析与检验医学福建省高校重点实验室,福建莆田351100

出　　处：《粮食与油脂》2022年第12期114-118,122,共6页Cereals & Oils

基　　金：福建省自然科学基金项目(2019J01806);莆田市科技计划项目(2018SP3004);莆田市科技局科研创新专项(2021ZP01);福建省中青年教师教育科研项目(JAT170512)。

摘　　要：以福鼎白茶为原料,在单因素的基础上采用正交试验优化福鼎白茶总黄酮的超声提取工艺,并测定白茶总黄酮的抗氧化能力。结果表明:超声提取福鼎白茶总黄酮的最佳工艺条件为乙醇体积分数60%、料液比1∶40(g/mL)、提取时间30 min、提取温度70℃、超声功率120 W,在此条件下总黄酮的得率为11.696%;白茶总黄酮具有较强的抗氧化能力,清除DPPH·和·OH的IC_(50)分别为0.011、0.043 mg/mL。Using Fuding white tea as raw material,the ultrasonic extraction technology of total flavonoids from Fuding white tea was optimized by orthogonal design based on the single factor experiment,and the antioxidant capacity of total flavonoids was determined.The results showed that the optimum extraction technology of total flavonoids from white tea were ethanol volume fraction 60%,solid-liquid ratio 1∶40(g/mL),extraction time 30 min,extraction temperature 70℃and ultrasonic power 120 W.Under these conditions,the yield of total flavonoids was 11.696%.The total flavonoids from white tea showed strong antioxidant capacity,and the IC_(50) of DPPH free radical and hydroxyl free radical were 0.011 mg/mL and 0.043 mg/mL,respectively.

关 键 词：白茶 总黄酮 超声提取 正交试验 抗氧化能力 

分 类 号：TS201.1[轻工技术与工程—食品科学]