基于高通量测序与培养基鉴定法分析不同省份不同养殖模式下小龙虾菌群的多样性  

Analysis of Microbial Diversity of Crayfish under Different Culture Modes in Different Provinces Based on High-throughput Sequencing and Traditional Culture Methods

作　　者：范鹏飞 冯武[1] 肖瑶 张月洁 杜红英 陈加平[1] FAN Pengfei;FENG Wu;XIAO Yao;ZHANG Yuejie;DU Hongying;CHEN Jiaping(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学与技术学院,湖北武汉430070

出　　处：《食品工业科技》2023年第1期181-188,共8页Science and Technology of Food Industry

基　　金：国家重点研发计划项目(2019YFC1606003)。

摘　　要：本研究以安徽霍邱、江苏盱眙、湖北荆州三地的小龙虾作为研究对象,在荆州另取稻虾互作和养殖塘两种模式的环境样本。利用高通量技术做菌相分析,利用培养基鉴定法进行分离纯化鉴定,验证优势菌种类和数量。结果表明:荆州两组小龙虾初始菌数略低于盱眙和霍邱小龙虾,选择培养基上差别较小。高通量测序结果表明,科水平上三地小龙虾的优势菌主要为气单胞菌科和肠杆菌科;稻虾塘土壤菌群丰度远高于养殖塘,水体菌群较相似。属水平上,荆州稻虾塘、养殖塘、盱眙和霍邱四组小龙虾样本菌群丰度存在差异,但气单胞菌属和不动杆菌属都占较高比例,依次为41.8%、1.8%,12.4%、11.4%,24.5%、27.5%,40.1%,13.0%。荆州稻虾塘小龙虾优势菌还包括乳球菌属和柠檬酸杆菌属,养殖塘样本存在哈夫尼亚菌属和乳球菌属,盱眙与霍邱小龙虾样本优势菌均有微小杆菌属和柠檬酸杆菌属。培养基结果表明三地小龙虾优势菌均主要为气单胞菌属、哈夫尼亚菌属和柠檬酸杆菌属,与高通量结果相符。In this study,crayfish from Huoqiu in Anhui Province,Xuyi in Jiangsu Province,and Jingzhou in Hubei Province were selected as the research objects.Environmental samples of two modes of rice-shrimp co-cropping and aquaculture ponds were taken from Jingzhou.High throughput technology was used to analyze the bacterial diversity,and culture-dependent method was used to isolate,purify and identify the bacteria to verify the type and quantities of dominant bacteria.The results showed that the initial bacterial count of Jingzhou crayfish was slightly lower than that of Xuyi and Huoqiu crayfish,and the difference in the selection medium was small.The results of high-throughput sequencing showed that the dominant of four samples groups bacteria were Aeromonadaceae and Enterobacteriaceae.The abundance of soil microbial community in rice shrimp pond was much higher than that in aquaculture pond,and the microbial community in water was similar.At the genus level,there were differences in the abundance of bacteria in four samples groups of crayfish samples from Jingzhou rice shrimp pond,aquaculture pond,Xuyi and Huoqiu,However,both Aeromonas and Acinetobacter accounted for a higher proportion,followed by 41.8%,1.8%,12.4%,11.4%,24.5%,27.5%,40.1%,13.0%.The dominant bacteria of crayfish in Jingzhou rice shrimp pond also included Lactococcus and Citrobacter,and the strains of Hafnia and Lactococcus were found in the aquaculture pond samples.The predominant bacteria in Xuyi and Huoqiu crayfish samples were Exiguobacterium and Citrobacter.The culture-dependent results showed that the dominant bacteria were Aeromonas,Hafnia and Citrobacter,which were consistent with the high-throughput results.

关 键 词：高通量测序 小龙虾菌群 优势菌种 环境菌群 稻虾共作 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]