基于高效液相色谱-质谱联用技术检测畜肉中的瓜尔胶  

Detection of Guar Gum in Adulterated Livestock Meat by High Performance Liquid Chromatography-Mass Spectrometry

作　　者：郭超 陈超[1] 李慧晨 王真 徐若琳 赵文涛[1] 李莹莹[1] 郭文萍[1,2] 张朝晖 GUO Chao;CHEN Chao;LI Huichen;WANG Zhen;XU Ruolin;ZHAO Wentao;LI Yingying;GUO Wenping;ZHANG Zhaohui(China Meat Research Center,Beijing 100068,China;Beijing Key Laboratory of Meat Processing Technology,Beijing 100068,China;Science and Technology Research Center of China Customs,Beijing 100026,China)

机构地区：[1]中国肉类食品综合研究中心,北京100068 [2]肉类加工技术北京市重点实验室,北京100068 [3]中国海关科学技术研究中心,北京100026

出　　处：《食品科学》2022年第24期349-356,共8页Food Science

基　　金：国家市场监督管理总局研究委托任务资助项目。

摘　　要：建立畜肉中瓜尔胶的高效液相色谱-质谱测定法。样品经β-甘露聚糖酶酶解,加入乙腈沉淀蛋白后离心,上清液经过中性氧化铝柱净化,用高效液相色谱-串联质谱仪测定,外标法定量。结果表明,瓜尔胶在线性范围为0~2 g/kg,线性相关系数在0.99以上,检出限为0.030 0 g/kg,定量限为0.100 g/kg,回收率为85.4%~109.8%,相对标准偏差在1.5%~9.3%之间。该方法前处理简便、检测指标明确、灵敏度高,可作为定量检测畜肉中添加瓜尔胶的有效手段。This study proposed a high performance liquid chromatography-mass spectrometry(HPLC-MS) method for the determination of guar gum in adulterated livestock meat.The samples were enzymatically hydrolyzed by β-mannanase,added with acetonitrile to precipitate protein,and then centrifuged.The supernatant was purified on a neutral alumina column,detected using a liquid chromatography-tandem mass spectrometer,and quantified by external standard method.The results showed that the linear range for guar gum was 0–2 g/kg,the correlation coefficient was above 0.99,the limit of detection(LOD) was 0.030 0 g/kg,the limit of quantification(LOQ) was 0.100 g/kg,and the recoveries ranged from 85.4%to 109.8%,with relative standard deviations(RSDs) between 1.5% and 9.3%.The developed method has the advantages of simple pretreatment,clear detection indexes and high sensitivity,and can be used as an effective method for detecting added guar gum in livestock meat.

关 键 词：注胶肉 瓜尔胶 高效液相色谱-质谱联用法 食品安全 

分 类 号：TS201.6[轻工技术与工程—食品科学]