气相色谱-内标法测定白酒中3种高级脂肪酸乙酯含量  

Determination of 3 higher fatty acid ethyl esters content in Baijiu by gas chromatography-internal standard method

作　　者：毕荣宇 乔敏莎 卢君 唐平 山其木格 王丽 王凡 李长文[1] BI Rongyu;QIAO Minsha;LU Jun;TANG Ping;SHAN Qimuge;WANG Li;WANG Fan;LI Changwen(Guizhou Guotai Liquor Group Co.,Ltd.,Zunyi 564501,China;Tasly Research Academy,Tianjin 300410,China)

机构地区：[1]贵州国台酒业集团股份有限公司,贵州遵义564501 [2]天士力控股集团有限公司研究院,天津300410

出　　处：《中国酿造》2022年第12期240-244,共5页China Brewing

基　　金：贵州省科技成果应用及产业化计划项目(黔科合成果[2020]2Y045);贵州省仁怀市科技计划项目(仁科支撑[2020]04号);遵义市科技计划项目(遵市科合R&D[2020]32号)。

摘　　要：以乙酸正戊酯为内标物,建立了白酒中棕榈酸乙酯、油酸乙酯和亚油酸乙酯的气相色谱-内标法检测方法,并对市售不同香型白酒、不同品牌酱香型白酒中3种高级脂肪酸乙酯的含量分布规律进行探究。结果表明,3种高级脂肪酸乙酯的分离效果良好,线性范围为0.300 0~0.400 0 mg/mL,相关系数(R^(2))均>0.999,精密度试验结果相对标准偏差(RSD)<5%,加标回收率为94.01%~116.97%,检出限为0.003 2~0.003 5 mg/mL,定量限为0.010 9~0.011 7 mg/mL。11种不同香型白酒样品的3种高级脂肪酸乙酯含量在0~0.062 2 mg/mL之间,其中,酱香型白酒含量最高(0.062 2 mg/mL);35种不同品牌酱香型白酒中3种高级脂肪酸乙酯总含量在0.003 5~0.075 8 mg/mL之间。该方法准确、灵敏度高,能对白酒中3种高级脂肪酸乙酯进行有效的分析,可用于酒类企业日常白酒分析检测。Using n-amyl acetate as internal standard, a method for the determination of ethyl palmitate, ethyl oleate and ethyl linoleate in Baijiu by gas chromatography-internal standard method was established. The content distribution of 3 kinds of higher fatty acid ethyl esters in Baijiu of different flavor types and sauce-flavor(Jiangxiangxing) Baijiu of different brands was also studied. The results showed that the separation effect of the 3 higher fatty acid ethyl esters was good, the linear range was 0.300 0-0.400 0 mg/ml, the correlation coefficient(R^(2)) was all greater than 0.999, the relative standard deviation(RSD) of precision test results was less than 5%, the spiked recovery was 94.01%-116.97%, the detection limit was 0.003 2-0.003 5 mg/ml,and the quantitative limit was 0.010 9-0.011 7 mg/ml. The contents of 3 higher fatty acid ethyl esters in 11 different flavor type Baijiu samples ranged from 0 to 0.062 2 mg/ml, among them, sauce-flavor Baijiu had the highest content(0.062 2 mg/ml). The total contents of 3 higher fatty acid ethyl esters in 35 different brands of sauce-flavor Baijiu were 0.003 5-0.075 8 mg/ml. The method was accurate and sensitive, and could be used for the effective analysis of 3 higher fatty acid ethyl esters in Baijiu and for the daily analysis and detection of Baijiu in liquor enterprises.

关 键 词：气相色谱 内标法 白酒 高级脂肪酸乙酯 检测 

分 类 号：TS261.7[轻工技术与工程—发酵工程]