Regulatory effects of evodiamine on glucose metabolism-related factors in CT26 colorectal carcinoma-bearing mice  

作　　者：Jiang-Yue Yu Kui Chen Ruo-Qin Zhao Li-Qun Wang Chao Gong Shen-Zhou Huang Yun-Juan Zheng Pei-Wen Zhu Xin-Ran Song Fang-Hua Wu 

机构地区：[1]General Surgery,Fujian Medical University Affiliated Fuzhou First Hospital,Fuzhou 350009,China [2]College of Forestry,Shanxi Agricultural University,Jinzhong 030801,China [3]School of Bioengineering,Tianjin Modern Vocational Technology College,Tianjin 300350,China

出　　处：《Precision Medicine Research》2022年第4期12-17,共6页精准医学研究

基　　金：the Affiliated Fuzhou First Hospital of Fujian Medical University,the Fuzhou Science and Technology planning project(2020-WS-123).

摘　　要：Objective:To investigate the therapeutic effect of evodiamine(EVO)on the expression of hexokinase(HK),lactate dehydrogenase A(LDHA),and pyruvate kinase M(PKM),key enzymes of glycolysis,in the tumor-bearing mice after modeling mouse colon cancer cells(CT26).Methods:A tumor-bearing mouse model was generated by administering axillary injection of CT26 and intraperitoneally injecting different doses of EVO.The therapeutic effects of EVO on CT26 tumor-bearing mice were evaluated by measuring the thymus and spleen indices,tumor volume,tumor suppression rate,and other related indicators in the tumor tissues of mice in each group after the administration of EVO,in addition,histopathological changes in the tumor tissues of the mice in the groups were studied by hematoxylin and eosin staining.The expression levels of HK,LDHA,and PKM in the tumor tissues of each group of mice were measured by performing Western blot to investigate the mechanism of EVO treatment in CT26 tumor-bearing mice.Results:EVO inhibited the growth of tumors in CT26-bearing mice and enhanced their splenic and thymic indices.Western blot results showed that EVO reduced the expression levels of HK,LDHA,and PKM proteins in the tumor tissues of CT26 tumor-bearing mice.Conclusion:EVO has a therapeutic effect on CT26 tumor-bearing mice,and its mechanism of action may be related to the low expression of key enzymes HK,LDHA and PKM of glycolysis in tumor tissues.

关 键 词：EVODIAMINE mouse colon cancer cells tumor-bearing mice GLYCOLYSIS HEXOKINASE lactate dehydrogenase A pyruvate kinase M 

分 类 号：R73[医药卫生—肿瘤]