梧桐子生物碱的提取及抗氧化、抑菌活性研究  

Study on Extraction, Antioxidant and Antibacterial Activities of Alkaloids from Chinese Parasol Seed

作　　者：王萍[1] 位治国[2] 郭金英[1] 任国艳[1] 吴影[1] 喻磊 WANG Ping;WEI Zhiguo;GUO Jinying;REN Guoyan;WU Ying;YU Lei(College of Food&Bioengineering,Henan University of Science and Technology,Luoyang 471023;College of Animal Science&Technology,Henan University of Science and Technology,Luoyang 471023)

机构地区：[1]河南科技大学食品与生物工程学院,洛阳471023 [2]河南科技大学动物科技学院,洛阳471023

出　　处：《食品工业》2022年第11期79-83,共5页The Food Industry

基　　金：河南省高等学校重点科研项目(15A180035);河南科技大学博士启动基金(09001660)。

摘　　要：研究采用超声辅助法,以乙醇体积分数、料液比、浸提温度和浸提时间为影响因素,提取率为评价指标,优化梧桐子生物碱的提取工艺;利用1, 1-二苯基-2-三硝基苯肼(1, 1-diphenyl-2-picrylhydrazyl, DPPH)、2, 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)[2, 2’-Azinobis-(3-ethylbenzthiazoline-6-sulphonate), ABTS]和总还原力评价梧桐子生物碱的抗氧化能力;并通过滤纸片法检测梧桐子生物碱的抑菌活性。结果表明:梧桐子生物碱提取的最佳条件为乙醇体积分数65%、料液比1∶20 g/mL、浸提温度40℃、浸提时间90 min,此时梧桐子生物碱提取率为4.02μg/mg;梧桐子生物碱具有较强的还原能力,并能有效地清除DPPH自由基和ABTS自由基,具有较强的抗氧化能力;梧桐子生物碱能有效地抑制大肠杆菌和金黄色葡萄球菌的生长,具有良好的抑菌活性。因此,梧桐子生物碱可作为一种新的天然抗氧化剂和抑菌剂。The ultrasonic-assisted extraction and optimization were studied by orthogonal experiment of alkaloids from Chinese parasol seed, with the ethanol concentration, ratio of material to liquid, extraction temperature and extraction time as the influencing factors and the extraction rate of total alkaloids as the evaluation index. Then, the antioxidant and antibacterial activities of the alkaloids by DPPH, ABTS and filter paper method were studied. The optimum extraction parameters of alkaloids from Chinese parasol seed were as follows: the ethanol concentration 65%, the ratio of material to liquid 1∶20 g/m L, the extraction temperature 40 ℃, and the extraction time 90 min. Under this technological condition, the extraction rate of alkaloids from Chinese parasol seed was 4.02 μg/mg. The strong reduction ability and the effective free radical scavenging ability for DPPH and ABTS showed that it had strong antioxidant capacity. In addition, the alkaloid of Chinese parasol seed could effective inhibit the growth of Escherichia coli and Staphylococcus aureus, indicating that it had a certain bacteriostatic activity. Therefore, the alkaloids of Chinese parasol seed could be used as a new natural antioxidant and bacteriostatic agent.

关 键 词：梧桐子 生物碱 超声波辅助提取 抗氧化 抑菌 

分 类 号：TS201.2[轻工技术与工程—食品科学]