产胞外谷胱甘肽酿酒酵母的诱变选育及发酵优化  

Breeding and Fermentation Optimization of Saccharomyces Cerevisiae Producing Extracellular Glutathione

作　　者：吴峥[1] 雷敏 蔡俊[1] WU Zheng;LEI Min;CAI Jun(Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei Key Laboratory of Industrial Microbiology,Hubei Provincial Cooperative Innovation Center of Industrial Faiermentation,Hubei University of Technology,Wuhan 430068,Hubei,China)

机构地区：[1]发酵工程教育部重点实验室,工业微生物湖北省重点实验室,工业发酵省部共建协同创新中心,湖北工业大学,湖北武汉430068

出　　处：《食品研究与开发》2023年第3期201-207,共7页Food Research and Development

摘　　要：为获得一株能产胞外谷胱甘肽(glutathione,GSH)的突变株,对酿酒酵母GAQ4进行紫外迭代诱变处理,最终筛选得到突变菌株UV3-10,其胞外谷胱甘肽含量为18.56 mg/L,是出发菌株GAQ4胞外谷胱甘肽含量的2.52倍。为进一步提高胞外谷胱甘肽含量,通过单因素试验优化突变株UV3-10发酵条件,得到其最优摇瓶发酵条件为发酵周期48 h、发酵温度34℃、摇瓶转速140 r/min、摇瓶装液量75 mL/300 m L、初始pH值为6.5;优化后其胞外GSH含量达到52.05 mg/L,是发酵条件优化前胞外GSH含量的2.80倍。To obtain a mutant strain that produces extracellular glutathione(GSH),Saccharmyces Cerevisiae GAQ4 was treated iteratively by UV mutagenesis,and a mutant strain named UV3-10 was finally screened after three times of UV mutagenesis. The extracellular GSH content of UV3-10 was 18.56 mg/L,which was 2.52 times that of original strain GAQ4. To further improve the extracellular GSH content,the fermentation conditions of mutant strain UV3-10 were optimized by single factor experiment. Results showed that the optimal fermentation conditions were 48 h fermentation cycle,fermentation temperature at 34 ℃,shake flask speed at 140 r/min,shake flask volume of 75 mL/300 mL,and initial pH of 6.5. Under these conditions,the extracellular GSH content reached 52.05 mg/L,which was 2.80 times of that before optimization,achieving the extracellular fermentation production of GSH.

关 键 词：酿酒酵母 谷胱甘肽 诱变筛选 胞外 发酵优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]