南极磷虾蛋白肽的抗氧化活性及稳定性研究  

Antioxidant Activity and Stability of Peptides from Antarctic Krill(Euphausia Superba)

作　　者：刘小芳[1] 黄岳磊 李雅婷 李福后 李爽 王灵昭 冷凯良[1,4] LIU Xiaofang;HUANG Yuelei;LI Yating;LI Fuhou;LI Shuang;WANG Lingzhao;LENG Kailiang(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fisheries,Ministry of Agriculture and Rural Affairs,Qingdao 266071,China;School of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005,China;National Engineering Research Center For Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,China;Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266200,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,农业农村部极地渔业可持续利用重点实验室,山东青岛266071 [2]江苏海洋大学食品科学与工程学院,江苏连云港222005 [3]浙江海洋大学,国家海洋设施养殖工程技术研究中心,浙江舟山316022 [4]青岛海洋科学与技术试点国家实验室,海洋药物与生物制品功能实验室,山东青岛266200

出　　处：《食品科技》2022年第11期114-120,共7页Food Science and Technology

基　　金：国家自然科学基金项目(32001770);国家重点研发计划项目(2020YFD0901204)。

摘　　要：目的:分析探讨南极磷虾蛋白肽的抗氧化活性及稳定性。方法:通过测定自由基清除能力以及脂质过氧化抑制能力,系统评价南极磷虾蛋白肽的抗氧化活性,并考察温度、酸碱度、模拟胃肠道消化等条件对其稳定性的影响。结果:南极磷虾蛋白肽对DPPH自由基、ABTS自由基、羟自由基和超氧阴离子自由基均具有较强的清除作用,IC_(50)分别为5.68、0.32、9.54、1.68 mg/mL;同时,南极磷虾蛋白肽对亚油酸、蛋黄卵磷脂等脂质过氧化具有良好的抑制作用。南极磷虾蛋白肽在温度>60℃条件下,抗氧化活性显著下降(P<0.05);在中性及弱酸性条件下较稳定,在碱性条件下抗氧化活性急剧下降(P<0.05);经体外模拟胃肠道消化后仍能保持较高的抗氧化活性。结论:南极磷虾蛋白肽具有较好的抗氧化活性,其相关产品的加工贮藏应避免高温、强酸、强碱条件的影响。研究结果可为南极磷虾蛋白肽的品质保持和相关高值产品开发提供科学指导。Objective:The antioxidant activity and stability of the Antarctic krill(Euphausia superba)peptides were investigated.Methods:Free radical scavenging capacity and lipid peroxidation inhibition ability of the Antarctic krill peptides were determined,while the effects of temperature,pH and simulated gastrointestinal digestion on the antioxidant activity of the Antarctic krill peptides were evaluated.Results:Antarctic krill peptides exhibited good scavenging abilities of the DPPH radical,ABTS radical,hydroxyl radical and superoxide anion radical with IC_(50)of 5.68,0.32,9.54,1.68 mg/mL,respectively.Antarctic krill peptides could inhibit the lipid peroxidation of linoleic acid and egg lecithin.High temperature(>60℃)had significant influence on the antioxidant activity of the Antarctic krill peptides(P<0.05).The antioxidant activity of the Antarctic krill peptides decreased sharply under alkaline conditions(P<0.05),which could maintain relatively stable under neutral and weak acidic conditions,as well as high activity after simulated gastrointestinal digestion in vitro.Conclusion:Antarctic krill peptides had good antioxidant activity,while the processing and storage of its related products should avoid the high temperature,strong acid and strong alkali conditions.The present research would provide scientific guidance for the quality maintenance and further application of the Antarctic krill peptides.

关 键 词：南极磷虾 蛋白肽 抗氧化活性 稳定性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]