丹江口库区土著Pediococcus pentosaceus菌株的分离鉴定及发酵培养基的优化  

Isolation,Identification and Optimization of Culture Medium for Indigenous Pediococcus pentosaceus in Danjiangkou Reservoir Area

作　　者：潘中闪 史红玲[2] 李莹[2] 李中洋 唐存多[2] 王娜[1] PAN Zhong-shan;SHI Hong-ling;LI Ying(College of Food Science and Technology,Henan Agricultural University,Zhengzhou,Henan 450002;Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province,Nanyang Normal University,Nanyang,Henan 473061)

机构地区：[1]河南农业大学食品科学技术学院,河南郑州450002 [2]南阳师范学院南水北调中线水源区水安全河南省协同创新中心,河南南阳473061 [3]恒利康生物科技股份有限公司,河南南阳473081

出　　处：《安徽农业科学》2023年第2期1-4,8,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31900916);河南省青年人才托举工程项目(2021HYTP036);南阳师范学院青年项目(2020QN003);河南省高校科技创新人才(21HASTIT041);河南省本科高校省级大学生创新创业训练计划项目(S202110481033)。

摘　　要：[目的]筛选出具有自主知识产权、良好适应库区环境的土著戊糖片球菌菌株,再通过对戊糖片球菌的发酵培养基进行条件优化,降低戊糖片球菌的发酵成本。[方法]采用MRS培养基从库区的青贮饲料中分离目的菌株,并进行16S rRNA的分子鉴定,再分别对戊糖片球菌发酵培养基中的碳源种类、碳源浓度、氮源种类、氮源浓度、无机盐离子及离子浓度进行优化。[结果]获得了1株具有自主知识产权的土著Pediococcus pentosaceus菌株,命名为Pediococcus pentosaceus NY001;优化得到较适宜的发酵培养基为葡萄糖40 g/L、酵母粉40 g/L、MgSO_(4)·7H_(2)O 1.0 g/L,在此条件下,戊糖片球菌的最大发酵活菌数可达2.2×10^(9) CFU/mL,显著高于初始发酵水平(2.5×10^(7)CFU/mL)的88倍。[结论]该研究为肠道益生菌的发酵培养及后续畜禽无抗健康养殖研究奠定了坚实基础。[Objective]To screen out the indigenous strains of Pediococcus pentosaceus with independent intellectual property rights and good adaptation to the environment of the reservoir area,and then to reduce the fermentation cost of Pediococcus pentosaceus by optimizing the fermentation medium.[Method]The target strains were isolated from silage using MRS medium and identified by 16S rRNA.And the main components of the medium were optimized respectively,including carbon source,carbon source concentration,nitrogen source,nitrogen source concentration,inorganic ions and ion concentration.[Result]A Pediococcus pentosaceus strain with independent intellectual property rights was obtained and named as Pediococcus pentosaceus NY001.The more suitable medium for the fermentation of P.pentosaceus NY001 was glucose 40 g/L,yeast powder 40 g/L and MgSO_(4)·7H_(2)O 1.0 g/L,under the fermentation conditions,the maximum number of viable bacteria could reach 2.2×10^(9) CFU/mL,which was about 88 times of the initial number of viable bacteria(2.5×10^(7) CFU/mL).[Conclusion]This study laid a solid foundation for the fermentation culture of intestinal probiotics and the subsequent research on the healthy breeding of livestock and poultry without anti bacteria.

关 键 词：戊糖片球菌 乳酸菌 菌种分离 培养基 发酵条件 优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]