DNA酶生物传感器在食源性致病菌检测中的研究进展  

Research Progress on DNAzyme Biosensor in the Detection of Foodborne Pathogens

作　　者：闫夏萌 张蕴哲[1] 卢鑫[2] 徐慧 张伟[1,3,4] 袁耀武[1] YAN Xia-meng;ZHANG Yun-zhe;LU Xin;XU Hui;ZHANG Wei;YUAN Yao-wu(College of Food Science and Technology,Hebei Agricultural University,Baoding 071001,Hebei,China;Department of Science and Engineering,Hebei Agricultural University,Cangzhou 061100,Hebei,China;College of Life Sciences,Hebei Agricultural University,Baoding 071001,Hebei,China;Key Laboratory of Analysis and Prevention and Control of Zoonosis in Hebei Province,Baoding 071000,Hebei,China)

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]河北农业大学理工系,河北沧州061100 [3]河北农业大学生命科学学院,河北保定071001 [4]河北省人畜共患病原微生物分析与防控重点实验室,河北保定071000

出　　处：《食品研究与开发》2023年第5期202-208,共7页Food Research and Development

基　　金：国家自然科学基金项目(32172288、31371772);河北省自然科学基金重点项目(C2019204342);中央引导地方科技发展资金项目(216Z5501G);河北省外专百人计划(360-0803-JSN-3YGS);河北农业大学食品加工学科群经费资助(2021-06)。

摘　　要：食源性致病菌是可以引起食物中毒或以食品为传播媒介的致病性细菌,其广泛存在于各类食品基质中,在食品加工运输过程中可以存活较长时间。人们摄入受污染食品会引发各种疾病,严重威胁人体健康。近年来,基于功能核酸的DNA酶生物传感器凭借其高稳定性、易于合成修饰、成本低廉等技术优势,其在致病菌检测领域得到广泛应用,并产生基于比色、荧光、电化学等致病菌检测技术。该文基于DNA酶生物传感器在食源性致病菌检测中的发展近况,对2种DNAzyme检测机制进行综述,并从分类及应用范围进行介绍,为食源性致病菌检测提供参考。Foodborne pathogens are pathogenic bacteria that can cause food poisoning or take food as the transmission medium,which widely exist in all kinds of food substrates and can survive for a long time during food processing and transportation. The ingestion of contaminated food can cause various diseases and seriously threaten human health. In recent years,the DNAzyme biosensor based on functional nucleic acid has been widely used in the field of pathogen detection because of its high stability,easy synthesis and modification,low cost and other technical advantages,which facilitates the development of pathogen detection technologies based on colorimetry,fluorescence and electrochemistry. Based on the research progress on the DNAzyme biosensor in the detection of foodborne pathogens in recent years,the present study reviewed two detection mechanisms of DNAzyme and introduced the classification and application of DNAzyme,aiming to provide references for the detection of foodborne pathogens.

关 键 词：食源性致病菌 DNA酶 生物传感器 检测技术 食品安全 

分 类 号：TS207.4[轻工技术与工程—食品科学]