西番莲果皮花色苷对H_(2)O_(2)诱导L02细胞氧化损伤的抑制作用  

Inhibition effect of peel of passion fruits anthocyanins on H_(2)O_(2)-induced oxidative damage in L02 cells

作　　者：张佳辰 丁蕾 何传波[1] 魏好程[1] 熊何健[1] ZHANG Jia-Chen;DING Lei;HE Chuan-Bo;WEI Hao-Cheng;XIONG He-Jian(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,厦门361021

出　　处：《食品安全质量检测学报》2023年第2期198-207,共10页Journal of Food Safety and Quality

基　　金：福建省区域发展项目(2019N3012);福建省科技计划项目(2019N0014)。

摘　　要：目的探究西番莲果皮花色苷(peel of passion fruits anthocyanins,PPFA)对H_(2)O_(2)诱导人正常肝细胞L02氧化损伤的抑制作用。方法采用噻唑蓝[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT]法建立H_(2)O_(2)诱导L02细胞氧化损伤的模型,通过测定细胞丙二醛(malondialdehyde,MDA)、乳酸脱氢酶(lactate dehydrogenase,LDH)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)指标,观察PPFA对细胞氧化损伤的抑制作用,结合非靶向代谢组学显著差异代谢物分析,探讨其抗氧化机制。结果PPFA体外清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的半抑制浓度(half maximal inhibitory concentration,IC_(50))值为34.62μg/m L,有一定的羟自由基、超氧阴离子自由基清除能力。细胞实验表明,PPFA能够显著提高H_(2)O_(2)损伤的L02细胞总抗氧化力(P<0.05),并显著降低损伤细胞中MDA生成量和LDH的释放量(P<0.05);与损伤组相比,PPFA各剂量组SOD活性均显著升高(P<0.05),中剂量组CAT与低剂量组GSH-Px的活性显著升高且达空白组水平(P<0.05)。代谢组学分析表明,与损伤组相比,PPFA处理后细胞存在23种显著差异代谢物,涉及到谷胱甘肽代谢、胆汁分泌、铁死亡和脂肪酸生物合成途径。结论PPFA对H_(2)O_(2)诱导L02细胞的氧化损伤有抑制作用,表现出显著的细胞抗氧化活性。Objective To investigate the inhibitory effect of peel of passion fruits anthocyanins(PPFA) on H_(2)O_(2)-induced oxidative damage in human normal liver cells L02. Methods A model of H_(2)O_(2)-induced oxidative damage in L02 cells was established by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)method,and the inhibitory effect of PPFA on cellular oxidative damage was observed by measuring cellular malondialdehyde(MDA),lactate dehydrogenase(LDH),superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px) indexes,combined with non-targeted metabolomics significantly different metabolite analysis to explore its antioxidant mechanism. Results The half maximal inhibitory concentration(IC_(50))value of 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging by PPFA in vitro was 34.62 μg/mL,and it had certain scavenging ability hydroxyl radicals and superoxide anion radicals. Cellular experiments showed that PPFA could significantly increase the total antioxidant power of H_(2)O_(2)-induced L02 cells(P<0.05),and significantly decrease the MDA production and LDH release in the damaged cells(P<0.05);compared with the damaged group,the SOD activity of PPFA was significantly increased in all dose groups(P<0.05),and the activity of CAT in the middle dose group and GSH-Px in the low dose group were significantly increased and reached the normal level(P<0.05). Metabolomic analysis showed that there were 23 kinds of significantly different metabolites in PPFA-treated cells compared with the damaged group,involving glutathione metabolism,bile secretion,ferroptosis and fatty acid biosynthesis pathways. Conclusion PPFA can inhibit the H_(2)O_(2)-induced oxidative damage in L02cells,showing significant cellular antioxidant activity.

关 键 词：西番莲果皮 花色苷 L02细胞 抗氧化活性 非靶向代谢组学 

分 类 号：TS209[轻工技术与工程—食品科学]