海产源多重耐药共生菌的耐药表型及耐药基因分析  

Analysis of antimicrobial resistance phenotype and genotype of multipleantimicrobial resistant commensal bacteria from marine products

作　　者：吴书香 李丽倩 姚琳[2] 王联珠[2] 曲梦 李风铃[2] 谭志军[2,3] 江艳华 王鹏[1] WU Shu-Xiang;LI Li-Qian;YAO Lin;WANG Lian-Zhu;QU Meng;LI Feng-Ling;TAN Zhi-Jun;JIANG Yan-Hua;WANG Peng(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Pilot National Laboratory for Marine Science and Technology,Qingdao 266033,China)

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003 [2]中国水产科学研究院黄海水产研究所农业农村部水产品质量安全检测与评价重点实验室,青岛266071 [3]青岛海洋科学与技术试点国家实验室,青岛266033

出　　处：《食品安全质量检测学报》2023年第2期248-255,共8页Journal of Food Safety and Quality

基　　金：中国水产科学研究院基本科研业务费项目(2020TD71);中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022020004)。

摘　　要：目的 对市售牡蛎和大黄鱼中分离的多重耐药共生菌进行耐药性分析、全基因组测序及耐药基因分析。方法 采用药敏纸片法进行耐药性分析,采用二代测序技术进行基因组框架图测序,通过与CARD数据库比对进行耐药基因分析。结果 菌株呈现高度的多重耐药性,对10种或10种以上药物耐药,多重耐药系数大于或等于0.48,所有菌株对头孢唑啉、萘啶酸、四环素、复方新诺明及氯霉素耐药。分离菌株为水产品中常见共生菌,基因组差异显著。菌株含有β-内酰胺类、氨基糖苷类、喹诺酮类、四环素类、磺胺类、氯霉素类、消毒剂类等药物的耐药基因,与耐药表型基本一致,部分菌含有对同一类药物具有不同耐药机制的多个基因。结论 牡蛎和大黄鱼中存在多重耐药共生菌,耐药基因丰富,如果耐药基因传播到致病菌会造成临床治疗失效,具有潜在的危害。Objective To analyze the antimicrobial resistance,whole genome sequence and antimicrobial resistance genes of multiple-antimicrobial resistant commensal bacteria isolated from commercially available oysters and large yellow croaker. Methods Antimicrobial resistance was performed by disk diffusion method,draft genome was sequenced by next-generation sequencing technology,and antimicrobial resistance genes were obtained by aligning the genome sequences to the CARD database. Results The strains showed a high degree of multiple resistance to 10 or more antimicrobials,with a multiple antimicrobial resistance index equal to or greater than 0.48. All strains were resistant to cefazoline,nalidixic acid,tetracycline,sulphamethoxazole/trimethoprim,and chloramphenicol.The isolates were commensal bacteria commonly found in aquatic products,and their genomic sequences were significantly different. The strains contained the resistance genes related to β-lactams,aminoglycosides,quinolones,tetracyclines,sulfonamides,chloramphenicol,disinfectants,etc.,which were basically consistent with the resistance phenotypes. Some strains had several genes with different resistance mechanisms for the same class of antimicrobial.Conclusion Multiple-antimicrobial resistant commensal bacteria are present in oysters and large yellow croaker and rich in lots of resistance genes. These bacteria and resistance genes may be the potential hazards if the resistance genes transfer to the pathogens and jeopardize the treatment of infections.

关 键 词：耐药性 耐药基因 共生菌 全基因组测序 牡蛎 大黄鱼 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]